TY - JOUR
T1 - Introduction to Section II
T2 - RNA methods to approach CFTR expression.
AU - Harris, Ann
PY - 2011
Y1 - 2011
N2 - In this section, we review methods for the analysis of the CFTR gene and its transcript. First, we discuss techniques to accurately measure levels of CFTR mRNA in primary human cells; next, protocols for measuring CFTR transcripts that contain premature termination codons and for evaluating the role of nonsense-mediated decay which targets these transcripts; a further chapter considers methodology to investigate pre-mRNA splicing. The penultimate chapter concentrates on methods for evaluating microRNA regulation of gene expression in the context of airway disease. The final chapter considers methods to evaluate the chromatin structure of the active CFTR locus and to analyse the cis-acting regulatory elements that control it.
AB - In this section, we review methods for the analysis of the CFTR gene and its transcript. First, we discuss techniques to accurately measure levels of CFTR mRNA in primary human cells; next, protocols for measuring CFTR transcripts that contain premature termination codons and for evaluating the role of nonsense-mediated decay which targets these transcripts; a further chapter considers methodology to investigate pre-mRNA splicing. The penultimate chapter concentrates on methods for evaluating microRNA regulation of gene expression in the context of airway disease. The final chapter considers methods to evaluate the chromatin structure of the active CFTR locus and to analyse the cis-acting regulatory elements that control it.
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U2 - 10.1007/978-1-61779-117-8_8
DO - 10.1007/978-1-61779-117-8_8
M3 - Editorial
C2 - 21594781
AN - SCOPUS:80052308961
SN - 1064-3745
VL - 741
SP - 111
EP - 113
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -