Inverse relationship between pilus-mediated gonococcal adherence and surface expression of the pilus receptor, CD46

D. M. Tobiason, H. S. Seifert*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Pilus-mediated adherence to mucosal epithelial cells is a critical step for Neisseria gonorrhoeae to establish an infection in the human host. CD46, the defined receptor for the gonococcal pilus, is a complement-regulatory protein that is expressed on all human nucleated cells. It was observed that a piliated, Opa- variant of gonococcal strain FA1090 adhered with different efficiencies to the human epithelial cell lines tested (Chang, ME180, HEC-1B and PC-3). Surprisingly, these differences in adherence levels did not correlate with levels of CD46 expressed by these cell lines. In fact, there was an inverse relationship between total surface-exposed CD46 and gonococcal adherence. Four major isoforms of CD46 are produced due to alternative RNA splicing of a surface-exposed region and the cytoplasmic tail. The relative isoform surface expression of each cell line was determined, and each was found to express different ratios of the four CD46 isoforms. No correlation could be derived between CD46 isoform surface expression and pilus-mediated gonococcal adherence, indicating that CD46 does not act as a classic receptor for gonococcal pili.

Original languageEnglish (US)
Pages (from-to)2333-2340
Number of pages8
JournalMicrobiology
Volume147
Issue number8
DOIs
StatePublished - 2001

Keywords

  • Flow cytometry
  • Fluorescent labelling
  • Human epithelial cells
  • Neisseria gonorrhoeae

ASJC Scopus subject areas

  • Microbiology

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