Many therapeutic natural products emanate from NRPS and PKS enzymes, and there is an ongoing effort to engineer these systems for the generation of novel natural products. Here, direct interrogation by Fourier-transform mass spectrometry is extended to 70-127 kDa polypeptides as a robust method to investigate these megaenzymes and facilitate deeper fundamental understanding and rational engineering efforts.
- Fourier-transfrom mass spectrometry
- Nonribosomal peptide synthesis
- Protein modifications
ASJC Scopus subject areas
- Molecular Medicine
- Molecular Biology
- Organic Chemistry