Investigating nonribosomal peptide and polyketide biosynthesis by direct detection of intermediates on > 70 kDa polypeptides by using Fourier-transform mass spectrometry

Leslie M. Hicks, Matthew T. Mazur, Leah M. Miller, Pieter C. Dorrestein, Nathan A. Schnarr, Chaitan Khosla, Neil L. Kelleher*

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Many therapeutic natural products emanate from NRPS and PKS enzymes, and there is an ongoing effort to engineer these systems for the generation of novel natural products. Here, direct interrogation by Fourier-transform mass spectrometry is extended to 70-127 kDa polypeptides as a robust method to investigate these megaenzymes and facilitate deeper fundamental understanding and rational engineering efforts.

Original languageEnglish (US)
Pages (from-to)904-907
Number of pages4
JournalChemBioChem
Volume7
Issue number6
DOIs
StatePublished - Jun 2006

Keywords

  • Biosynthesis
  • Fourier-transfrom mass spectrometry
  • Nonribosomal peptide synthesis
  • Polyketides
  • Protein modifications

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

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