Investigating the nucleic acid interactions and antimicrobial mechanism of buforin II

Erika T. Uyterhoeven, Chase H. Butler, Danette Ko, Donald E. Elmore*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

65 Scopus citations

Abstract

Buforin II (BF2) is an antimicrobial peptide that is hypothesized to kill bacteria by entering cells and binding nucleic acids. To further investigate this proposed mechanism, we used computer modeling and experimental measurements to consider the interactions between BF2 and DNA. Computational and experimental results imply that the peptide forms specific interactions with DNA. Moreover, we observe a general correlation between DNA affinity and antimicrobial activity for a series of BF2 variants. Thus, our results support the proposed mechanism for BF2 and provide a useful approach for evaluating the nucleic acid interactions of other antimicrobial peptides.

Original languageEnglish (US)
Pages (from-to)1715-1718
Number of pages4
JournalFEBS Letters
Volume582
Issue number12
DOIs
StatePublished - May 28 2008

Keywords

  • Buforin II
  • Hipposin
  • Histone-derived antimicrobial peptide
  • Molecular dynamics
  • Parasin
  • Peptide-DNA interaction

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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