Investigating the nucleic acid interactions and antimicrobial mechanism of buforin II

Erika T. Uyterhoeven; Chase H. Butler; Danette Ko; Donald E. Elmore

doi:10.1016/j.febslet.2008.04.036

Investigating the nucleic acid interactions and antimicrobial mechanism of buforin II

Erika T. Uyterhoeven, Chase H. Butler, Danette Ko, Donald E. Elmore^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

78 Scopus citations

Abstract

Buforin II (BF2) is an antimicrobial peptide that is hypothesized to kill bacteria by entering cells and binding nucleic acids. To further investigate this proposed mechanism, we used computer modeling and experimental measurements to consider the interactions between BF2 and DNA. Computational and experimental results imply that the peptide forms specific interactions with DNA. Moreover, we observe a general correlation between DNA affinity and antimicrobial activity for a series of BF2 variants. Thus, our results support the proposed mechanism for BF2 and provide a useful approach for evaluating the nucleic acid interactions of other antimicrobial peptides.

Original language	English (US)
Pages (from-to)	1715-1718
Number of pages	4
Journal	FEBS Letters
Volume	582
Issue number	12
DOIs	https://doi.org/10.1016/j.febslet.2008.04.036
State	Published - May 28 2008
Externally published	Yes

Funding

We thank Chrystle Cu and Caroline Ong for assistance in initial data collection and useful discussions. This work was supported by a Cottrell College award from Research Corporation and Wellesley College, with additional student support from NSF-REU Grant CHE-0353813.

Keywords

Buforin II
Hipposin
Histone-derived antimicrobial peptide
Molecular dynamics
Parasin
Peptide-DNA interaction

ASJC Scopus subject areas

Genetics
Molecular Biology
Biophysics
Structural Biology
Biochemistry
Cell Biology

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10.1016/j.febslet.2008.04.036

Cite this

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title = "Investigating the nucleic acid interactions and antimicrobial mechanism of buforin II",

abstract = "Buforin II (BF2) is an antimicrobial peptide that is hypothesized to kill bacteria by entering cells and binding nucleic acids. To further investigate this proposed mechanism, we used computer modeling and experimental measurements to consider the interactions between BF2 and DNA. Computational and experimental results imply that the peptide forms specific interactions with DNA. Moreover, we observe a general correlation between DNA affinity and antimicrobial activity for a series of BF2 variants. Thus, our results support the proposed mechanism for BF2 and provide a useful approach for evaluating the nucleic acid interactions of other antimicrobial peptides.",

keywords = "Buforin II, Hipposin, Histone-derived antimicrobial peptide, Molecular dynamics, Parasin, Peptide-DNA interaction",

author = "Uyterhoeven, {Erika T.} and Butler, {Chase H.} and Danette Ko and Elmore, {Donald E.}",

note = "Funding Information: We thank Chrystle Cu and Caroline Ong for assistance in initial data collection and useful discussions. This work was supported by a Cottrell College award from Research Corporation and Wellesley College, with additional student support from NSF-REU Grant CHE-0353813. ",

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doi = "10.1016/j.febslet.2008.04.036",

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AU - Uyterhoeven, Erika T.

AU - Butler, Chase H.

AU - Ko, Danette

AU - Elmore, Donald E.

N1 - Funding Information: We thank Chrystle Cu and Caroline Ong for assistance in initial data collection and useful discussions. This work was supported by a Cottrell College award from Research Corporation and Wellesley College, with additional student support from NSF-REU Grant CHE-0353813.

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Y1 - 2008/5/28

N2 - Buforin II (BF2) is an antimicrobial peptide that is hypothesized to kill bacteria by entering cells and binding nucleic acids. To further investigate this proposed mechanism, we used computer modeling and experimental measurements to consider the interactions between BF2 and DNA. Computational and experimental results imply that the peptide forms specific interactions with DNA. Moreover, we observe a general correlation between DNA affinity and antimicrobial activity for a series of BF2 variants. Thus, our results support the proposed mechanism for BF2 and provide a useful approach for evaluating the nucleic acid interactions of other antimicrobial peptides.

AB - Buforin II (BF2) is an antimicrobial peptide that is hypothesized to kill bacteria by entering cells and binding nucleic acids. To further investigate this proposed mechanism, we used computer modeling and experimental measurements to consider the interactions between BF2 and DNA. Computational and experimental results imply that the peptide forms specific interactions with DNA. Moreover, we observe a general correlation between DNA affinity and antimicrobial activity for a series of BF2 variants. Thus, our results support the proposed mechanism for BF2 and provide a useful approach for evaluating the nucleic acid interactions of other antimicrobial peptides.

KW - Buforin II

KW - Hipposin

KW - Histone-derived antimicrobial peptide

KW - Molecular dynamics

KW - Parasin

KW - Peptide-DNA interaction

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Investigating the nucleic acid interactions and antimicrobial mechanism of buforin II

Abstract

Funding

Keywords

ASJC Scopus subject areas

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