Recent success in assisted fertilization mainly depended on the development of sperm microinjection methods: intracytoplasmic sperm injection and subzonal insemination. Some basic mechanisms that under-lie fertilization were revealed by using intracytoplasmic sperm injection. In respect to this, problems of fertility, oocyte activation, formation of pronuclei and practical aspects of intracytoplasmic sperm injection are discussed.
|Original language||English (US)|
|Number of pages||9|
|State||Published - Dec 1 2000|
- Assisted fertilization
- Oocyte activation
- Sperm microinjection
ASJC Scopus subject areas