Investigation of respiratory syncytial virus outbreak on an adult stem cell transplant unit by use of whole-genome sequencing

Yijun Zhu*, Teresa R Zembower, Kristen E. Metzger, Zhengdeng Lei, Stefan J. Green, Chao Qi

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

A viral whole-genome sequencing (WGS) strategy, based on PCR amplification followed by next-generation sequencing, was used to investigate a nosocomial respiratory syncytial virus-B (RSV-B) outbreak in a hematology-oncology and stem cell transplant unit. RSV-B genomes from 16 patients and health care workers (HCWs) suspected to be involved in the outbreak were compared to RSV-B genomes that were acquired from outpatients during the same time period but epidemiologically unrelated to the outbreak. Phylogenetic analysis of the whole genome identified a cluster of 11 patients and HCWs who had an identical RSV-B strain which was clearly distinct from strains recovered from individuals unrelated to the outbreak. Sequence variation of the glycoprotein (G) gene alone was insufficient to distinguish the outbreak strains from the outbreak-unrelated strains, thereby demonstrating that WGS is valuable for local outbreak investigation.

Original languageEnglish (US)
Pages (from-to)2956-2963
Number of pages8
JournalJournal of Clinical Microbiology
Volume55
Issue number10
DOIs
StatePublished - Oct 1 2017

Keywords

  • Respiratory syncytial virus
  • Whole-genome sequencing

ASJC Scopus subject areas

  • Microbiology (medical)

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