TY - JOUR
T1 - Investigation of the Bcl-2 multimerisation process
T2 - Structural and functional implications
AU - Camperchioli, Alessia
AU - Mariani, Marisa
AU - Bartollino, Silvia
AU - Petrella, Lella
AU - Persico, Marco
AU - Orteca, Nausicaa
AU - Scambia, Giovanni
AU - Shahabi, Shohreh
AU - Ferlini, Cristiano
AU - Fattorusso, Caterina
N1 - Funding Information:
This work was partially supported by a grant coming from Fondazione Banco di Napoli .
PY - 2011/5
Y1 - 2011/5
N2 - Bcl-2 plays a prominent role in regulating the function of mitochondria during respiration and in determining the threshold of apoptotic sensitivity. Despite its relevance, the mechanism through which these processes are achieved is still unknown. Using surface plasmon resonance technology to monitor Bcl-2 multimerisation we discovered that a simple dimeric model does not fit with experimental data. A molecular model of the experimentally observed Bcl-2 homomeric complex has been developed. Accordingly, using a panel of mutants we identified in the loop a critical region for the process of Bcl-2 multimerisation. Our results indicate that the Bcl-2 loop posttranscriptional changes can modulate its ability to make homo and hetero-complexes, ultimately leading to functional modulation, suggesting an intriguing relationship between the ability of Bcl-2 to form multimeric complexes and its multi-functional role as a membrane channel. This article is part of a Special Issue entitled: 11th European Symposium on Calcium.
AB - Bcl-2 plays a prominent role in regulating the function of mitochondria during respiration and in determining the threshold of apoptotic sensitivity. Despite its relevance, the mechanism through which these processes are achieved is still unknown. Using surface plasmon resonance technology to monitor Bcl-2 multimerisation we discovered that a simple dimeric model does not fit with experimental data. A molecular model of the experimentally observed Bcl-2 homomeric complex has been developed. Accordingly, using a panel of mutants we identified in the loop a critical region for the process of Bcl-2 multimerisation. Our results indicate that the Bcl-2 loop posttranscriptional changes can modulate its ability to make homo and hetero-complexes, ultimately leading to functional modulation, suggesting an intriguing relationship between the ability of Bcl-2 to form multimeric complexes and its multi-functional role as a membrane channel. This article is part of a Special Issue entitled: 11th European Symposium on Calcium.
KW - Apoptosis
KW - Bcl-2 structure/function
KW - Multimeric protein complexes
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U2 - 10.1016/j.bbamcr.2011.02.006
DO - 10.1016/j.bbamcr.2011.02.006
M3 - Article
C2 - 21320534
AN - SCOPUS:79955654086
SN - 0167-4889
VL - 1813
SP - 850
EP - 857
JO - Biochimica et Biophysica Acta - Molecular Cell Research
JF - Biochimica et Biophysica Acta - Molecular Cell Research
IS - 5
ER -