Involvement of acid-sensing ion channel 1a in gastric carcinoma cell migration and invasion

Xin Chen, Xue Sun, Zhe Wang, Xiaojun Zhou, Lu Xu, Feng'E Li, Xingding Zhang, Ji'An Pan, Lin Qi, Haixin Qian, Zhongqi Mao*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Acidic microenvironment, particularly acid-sensing ion channel 1a (ASIC1a), has been reported to promote carcinoma cell proliferation as well as migration. In this study, we explored the effect of ASIC1a on migration and invasion of gastric carcinoma (GC). ASIC1a expression levels were examined in paired GC and adjacent normal tissues from 16 patients by immunohistochemistry. Reverse transcription real-time PCR and immunoblotting were conducted to assess the ASIC1a expression levels in the GC cell line AGS after transfection with ASIC1a small hairpin RNA (shRNA). Wound healing and transwell invasion assays were utilized to detect metastasis and invasion following ASIC1a silencing. Tumor formation was used to detect the role of ASIC1a in tumorigenicity in vivo. It was found that ASIC1a expression level was significantly higher in GC tissues showing postoperative metastasis compared with non-metastasis and non-tumor tissues. Moreover, silencing of ASIC1a with shRNA significantly down-regulated ASIC1a expression and reduced GC cell migration and invasion. A moderately acidic extracellular environment inhibited GC cell viability. Furthermore, ASIC1a shRNA caused inhibition of tumorigenicity in vivo. Our study is the first report of attenuating the malignant phenotype of GC in vitro and in vivo by suppressing ASIC1a, and suggests a novel approach to study the relationship between ASICs and GC cell migration and invasion.

Original languageEnglish (US)
Pages (from-to)440-446
Number of pages7
JournalActa biochimica et biophysica Sinica
Volume50
Issue number5
DOIs
StatePublished - May 1 2018

Fingerprint

Acid Sensing Ion Channels
Cell Movement
Stomach
Cells
Carcinoma
Small Interfering RNA
Tissue
Neoplasm Metastasis
Cell proliferation
Transcription
Application specific integrated circuits
Immunoblotting
Wound Healing

Keywords

  • acid-sensing ion channel 1a
  • gastric carcinoma
  • invasion
  • migration

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

Chen, Xin ; Sun, Xue ; Wang, Zhe ; Zhou, Xiaojun ; Xu, Lu ; Li, Feng'E ; Zhang, Xingding ; Pan, Ji'An ; Qi, Lin ; Qian, Haixin ; Mao, Zhongqi. / Involvement of acid-sensing ion channel 1a in gastric carcinoma cell migration and invasion. In: Acta biochimica et biophysica Sinica. 2018 ; Vol. 50, No. 5. pp. 440-446.
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abstract = "Acidic microenvironment, particularly acid-sensing ion channel 1a (ASIC1a), has been reported to promote carcinoma cell proliferation as well as migration. In this study, we explored the effect of ASIC1a on migration and invasion of gastric carcinoma (GC). ASIC1a expression levels were examined in paired GC and adjacent normal tissues from 16 patients by immunohistochemistry. Reverse transcription real-time PCR and immunoblotting were conducted to assess the ASIC1a expression levels in the GC cell line AGS after transfection with ASIC1a small hairpin RNA (shRNA). Wound healing and transwell invasion assays were utilized to detect metastasis and invasion following ASIC1a silencing. Tumor formation was used to detect the role of ASIC1a in tumorigenicity in vivo. It was found that ASIC1a expression level was significantly higher in GC tissues showing postoperative metastasis compared with non-metastasis and non-tumor tissues. Moreover, silencing of ASIC1a with shRNA significantly down-regulated ASIC1a expression and reduced GC cell migration and invasion. A moderately acidic extracellular environment inhibited GC cell viability. Furthermore, ASIC1a shRNA caused inhibition of tumorigenicity in vivo. Our study is the first report of attenuating the malignant phenotype of GC in vitro and in vivo by suppressing ASIC1a, and suggests a novel approach to study the relationship between ASICs and GC cell migration and invasion.",
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Chen, X, Sun, X, Wang, Z, Zhou, X, Xu, L, Li, FE, Zhang, X, Pan, JA, Qi, L, Qian, H & Mao, Z 2018, 'Involvement of acid-sensing ion channel 1a in gastric carcinoma cell migration and invasion', Acta biochimica et biophysica Sinica, vol. 50, no. 5, pp. 440-446. https://doi.org/10.1093/abbs/gmy026

Involvement of acid-sensing ion channel 1a in gastric carcinoma cell migration and invasion. / Chen, Xin; Sun, Xue; Wang, Zhe; Zhou, Xiaojun; Xu, Lu; Li, Feng'E; Zhang, Xingding; Pan, Ji'An; Qi, Lin; Qian, Haixin; Mao, Zhongqi.

In: Acta biochimica et biophysica Sinica, Vol. 50, No. 5, 01.05.2018, p. 440-446.

Research output: Contribution to journalArticle

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T1 - Involvement of acid-sensing ion channel 1a in gastric carcinoma cell migration and invasion

AU - Chen, Xin

AU - Sun, Xue

AU - Wang, Zhe

AU - Zhou, Xiaojun

AU - Xu, Lu

AU - Li, Feng'E

AU - Zhang, Xingding

AU - Pan, Ji'An

AU - Qi, Lin

AU - Qian, Haixin

AU - Mao, Zhongqi

PY - 2018/5/1

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