Involvement of cell cycle progression in survival signaling through CD40 in the B-lymphocyte line WEHI-231

H. Hirai, T. Adachi, T. Tsubata*

*Corresponding author for this work

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The CD40 molecule transmits a signal that abrogates apoptosis induced by ligation of the antigen receptor (BCR) in both primary B cells and B-cell lines such as WEHI-231. Expression of Bcl-xL and A1, antiapoptotic members of the Bcl-2 family, is enhanced by CD40 ligation, and is suggested to mediate CD40-induced B-cell survival. CD40 ligation also promotes cell cycle progression by increasing the levels of cyclin-dependent kinases (CDKs) required for cell cycle progression, and reducing expression of the CDK inhibitor p27kip1. Here we demonstrate that cell cycle inhibition by retrovirus-mediated p27kip1 expression does not modulate the levels of Bcl-xL or A1, but significantly reduces the survival of BCR-ligated WEHI-231 cells by CD40 ligation. This indicates that cell cycle progression is crucial for CD40-mediated survival of B cells.

Original languageEnglish (US)
Pages (from-to)261-269
Number of pages9
JournalCell Death and Differentiation
Volume11
Issue number3
DOIs
StatePublished - Mar 1 2004

Keywords

  • A1
  • Apoptosis
  • B Lymphocyte
  • Bcl-xL
  • CD40
  • Cell cycle progression
  • p27

ASJC Scopus subject areas

  • Cell Biology

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