Involvement of cell cycle progression in survival signaling through CD40 in the B-lymphocyte line WEHI-231

H. Hirai; T. Adachi; T. Tsubata

doi:10.1038/sj.cdd.4401357

Involvement of cell cycle progression in survival signaling through CD40 in the B-lymphocyte line WEHI-231

H. Hirai, T. Adachi, T. Tsubata^*

^*Corresponding author for this work

Pediatrics

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The CD40 molecule transmits a signal that abrogates apoptosis induced by ligation of the antigen receptor (BCR) in both primary B cells and B-cell lines such as WEHI-231. Expression of Bcl-xL and A1, antiapoptotic members of the Bcl-2 family, is enhanced by CD40 ligation, and is suggested to mediate CD40-induced B-cell survival. CD40 ligation also promotes cell cycle progression by increasing the levels of cyclin-dependent kinases (CDKs) required for cell cycle progression, and reducing expression of the CDK inhibitor p27^kip1. Here we demonstrate that cell cycle inhibition by retrovirus-mediated p27^kip1 expression does not modulate the levels of Bcl-xL or A1, but significantly reduces the survival of BCR-ligated WEHI-231 cells by CD40 ligation. This indicates that cell cycle progression is crucial for CD40-mediated survival of B cells.

Original language	English (US)
Pages (from-to)	261-269
Number of pages	9
Journal	Cell Death and Differentiation
Volume	11
Issue number	3
DOIs	https://doi.org/10.1038/sj.cdd.4401357
State	Published - Mar 2004

Keywords

A1
Apoptosis
B Lymphocyte
Bcl-xL
CD40
Cell cycle progression
p27

ASJC Scopus subject areas

Molecular Biology
Cell Biology

Access to Document

10.1038/sj.cdd.4401357

Cite this

@article{e4b67fce9c584204a3d9b3fa3fc68d24,

title = "Involvement of cell cycle progression in survival signaling through CD40 in the B-lymphocyte line WEHI-231",

abstract = "The CD40 molecule transmits a signal that abrogates apoptosis induced by ligation of the antigen receptor (BCR) in both primary B cells and B-cell lines such as WEHI-231. Expression of Bcl-xL and A1, antiapoptotic members of the Bcl-2 family, is enhanced by CD40 ligation, and is suggested to mediate CD40-induced B-cell survival. CD40 ligation also promotes cell cycle progression by increasing the levels of cyclin-dependent kinases (CDKs) required for cell cycle progression, and reducing expression of the CDK inhibitor p27kip1. Here we demonstrate that cell cycle inhibition by retrovirus-mediated p27kip1 expression does not modulate the levels of Bcl-xL or A1, but significantly reduces the survival of BCR-ligated WEHI-231 cells by CD40 ligation. This indicates that cell cycle progression is crucial for CD40-mediated survival of B cells.",

keywords = "A1, Apoptosis, B Lymphocyte, Bcl-xL, CD40, Cell cycle progression, p27",

author = "H. Hirai and T. Adachi and T. Tsubata",

note = "Funding Information: We thank Drs. Y Kitamura, A Miyajima, Y Terada (Tokyo Medical and Dental University), E Harlow and H Yagita for reagents and Mr. K Mizuno for technical help. This work was supported by grants from the Ministry of Education, Culture, Sport, Science and Technology, Ministry of Health, Labor and Welfare of Japan.",

year = "2004",

month = mar,

doi = "10.1038/sj.cdd.4401357",

language = "English (US)",

volume = "11",

pages = "261--269",

journal = "Cell Death and Differentiation",

issn = "1350-9047",

publisher = "Nature Publishing Group",

number = "3",

}

TY - JOUR

T1 - Involvement of cell cycle progression in survival signaling through CD40 in the B-lymphocyte line WEHI-231

AU - Hirai, H.

AU - Adachi, T.

AU - Tsubata, T.

N1 - Funding Information: We thank Drs. Y Kitamura, A Miyajima, Y Terada (Tokyo Medical and Dental University), E Harlow and H Yagita for reagents and Mr. K Mizuno for technical help. This work was supported by grants from the Ministry of Education, Culture, Sport, Science and Technology, Ministry of Health, Labor and Welfare of Japan.

PY - 2004/3

Y1 - 2004/3

N2 - The CD40 molecule transmits a signal that abrogates apoptosis induced by ligation of the antigen receptor (BCR) in both primary B cells and B-cell lines such as WEHI-231. Expression of Bcl-xL and A1, antiapoptotic members of the Bcl-2 family, is enhanced by CD40 ligation, and is suggested to mediate CD40-induced B-cell survival. CD40 ligation also promotes cell cycle progression by increasing the levels of cyclin-dependent kinases (CDKs) required for cell cycle progression, and reducing expression of the CDK inhibitor p27kip1. Here we demonstrate that cell cycle inhibition by retrovirus-mediated p27kip1 expression does not modulate the levels of Bcl-xL or A1, but significantly reduces the survival of BCR-ligated WEHI-231 cells by CD40 ligation. This indicates that cell cycle progression is crucial for CD40-mediated survival of B cells.

AB - The CD40 molecule transmits a signal that abrogates apoptosis induced by ligation of the antigen receptor (BCR) in both primary B cells and B-cell lines such as WEHI-231. Expression of Bcl-xL and A1, antiapoptotic members of the Bcl-2 family, is enhanced by CD40 ligation, and is suggested to mediate CD40-induced B-cell survival. CD40 ligation also promotes cell cycle progression by increasing the levels of cyclin-dependent kinases (CDKs) required for cell cycle progression, and reducing expression of the CDK inhibitor p27kip1. Here we demonstrate that cell cycle inhibition by retrovirus-mediated p27kip1 expression does not modulate the levels of Bcl-xL or A1, but significantly reduces the survival of BCR-ligated WEHI-231 cells by CD40 ligation. This indicates that cell cycle progression is crucial for CD40-mediated survival of B cells.

KW - A1

KW - Apoptosis

KW - B Lymphocyte

KW - Bcl-xL

KW - CD40

KW - Cell cycle progression

KW - p27

UR - http://www.scopus.com/inward/record.url?scp=1542359581&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1542359581&partnerID=8YFLogxK

U2 - 10.1038/sj.cdd.4401357

DO - 10.1038/sj.cdd.4401357

M3 - Article

C2 - 14647241

AN - SCOPUS:1542359581

SN - 1350-9047

VL - 11

SP - 261

EP - 269

JO - Cell Death and Differentiation

JF - Cell Death and Differentiation

IS - 3

ER -

Involvement of cell cycle progression in survival signaling through CD40 in the B-lymphocyte line WEHI-231

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this