Ion mobility separation of variant histone tails extending to the "middle-down" range

Alexandre A. Shvartsburg*, Yupeng Zheng, Richard D. Smith, Neil L. Kelleher

*Corresponding author for this work

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Differential ion mobility spectrometry (FAIMS) can baseline-resolve multiple variants of post-translationally modified peptides extending to the 3-4 kDa range, which differ in the localization of a PTM as small as acetylation. Essentially orthogonal separations for different charge states expand the total peak capacity with the number of observed states that increases for longer polypeptides. This potentially enables resolving localization variants for yet larger peptides and even intact proteins.

Original languageEnglish (US)
Pages (from-to)4271-4276
Number of pages6
JournalAnalytical Chemistry
Volume84
Issue number10
DOIs
StatePublished - May 15 2012

ASJC Scopus subject areas

  • Analytical Chemistry

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