Ion trap tandem mass spectrometry applied to small multiply charged oligonucleotides with a modified base

Scott A. McLuckey*, Sohrab Habibi-Goudarzi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

Two isomeric oligodeoxynucleotide hexamers, 5′-d(N-6meATGCAT)-3′ and 5′-d(ATGSmeCAT)-3′, were subjected to analysis by electrospray and ion trap mass spectrometry. In the case of the isomer with a modified adenine, location of the modified base in the sequence was straightforward and a triple mass spectrometry experiment provided information on the identity of the modification. In contrast, the isomer with the methylated cytosine did not yield definitive information on the location or identity of the modification. Tandem mass spectrometry data in this case could indicate that the modification was present on either the third or fourth nucleoside. The two isomers represent extremes in the facility with which modified bases can be identified and located in a small oligonucleotide via multiple mass spectrometry of multiply charged anions. A preference for loss of particular bases strongly influences which structurally diagnostic ions are formed upon collisional activation. The likelihood for locating and identifying a modified base is dependent, therefore, upon the likelihood that the base is lost directly from the parention.

Original languageEnglish (US)
Pages (from-to)740-747
Number of pages8
JournalJournal of the American Society for Mass Spectrometry
Volume5
Issue number8
DOIs
StatePublished - Aug 1994

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

Fingerprint

Dive into the research topics of 'Ion trap tandem mass spectrometry applied to small multiply charged oligonucleotides with a modified base'. Together they form a unique fingerprint.

Cite this