Ionic mechanism of action of a spin-labeled local anesthetic on squid axon membranes

J. Z. Yeh*, Kazu Takeno, Gerald M. Rosen, Toshio Narahashi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The ionic mechanism of action of a spin-labeled local anesthetic (SLA), 2-[N-methyl-N-(2,2,6,6-tetramethylpiperidinooxyl)]-ethyl 4-ethoxylbenzoate, was studied by means of voltage clamp technique with squid giant axons in comparison with the parent compound without spin label moiety, 2-(N,N-dimethyl)ethyl 4-ethoxylbenzoate (GS-01). Like other local anesthetics, they suppressed both sodium and potassium conductance increases. However, three remarkable differences have been noted between SLA and GS-01: (1) SLA is more effective than GS-01 in suppressing the sodium and potassium conductance increases; (2) SLA induces a potassium inactivation, whereas GS-01 is lacking this ability; (3) SLA has no effect on the time to peak sodium current, whereas GS-01 prolongs it. GS-01 resembles procaine with respect to (2) and (3) above. SLA will become a useful probe for the study of the molecular mechanism of local anesthetic action and of ionic channel function.

Original languageEnglish (US)
Pages (from-to)237-247
Number of pages11
JournalThe Journal of Membrane Biology
Volume25
Issue number1
DOIs
StatePublished - Dec 1975

ASJC Scopus subject areas

  • Biophysics
  • Physiology
  • Cell Biology

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