Ionophore A23187 promotes osteoclast formation in bone organ culture

P. H. Stern; M. F. Orr; E. Brull

doi:10.1007/BF02411205

Ionophore A23187 promotes osteoclast formation in bone organ culture

P. H. Stern^*, M. F. Orr, E. Brull

^*Corresponding author for this work

Pharmacology

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Morphologic effects of the ionophore A23187 were compared with those of PTH and 1,25(OH)₂ vitamin D₃ on fetal rat limb bone cultures. All three treatments resulted in loss of bone and the appearance of multinucleate osteoclasts. Only PTH caused proliferation of fibroblast-like cells within the marrow cavity. Cleanly dissected ("stripped") bones were unresponsive to A23187 and exhibited many dead and dying cells after 48 h in culture with this agent; in contrast, stripped bones were responsive to PTH. All three treatments showed a similar time course of appearance of osteoclasts. The results indicate that all three agents promote osteoclast formation. There are several possible explanations for the differential effects of A23187 and PTH in stripped bones, including the possibility that the cells of the periosteum are required for the action of A23187 but not of PTH.

Original language	English (US)
Pages (from-to)	31-36
Number of pages	6
Journal	Calcified Tissue International
Volume	34
Issue number	1
DOIs	https://doi.org/10.1007/BF02411205
State	Published - Dec 1 1982

Keywords

A23187
Bone resorption
Ionophore
Osteoclasts

ASJC Scopus subject areas

Endocrinology, Diabetes and Metabolism
Orthopedics and Sports Medicine
Endocrinology

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10.1007/BF02411205

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AU - Stern, P. H.

AU - Orr, M. F.

AU - Brull, E.

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N2 - Morphologic effects of the ionophore A23187 were compared with those of PTH and 1,25(OH)2 vitamin D3 on fetal rat limb bone cultures. All three treatments resulted in loss of bone and the appearance of multinucleate osteoclasts. Only PTH caused proliferation of fibroblast-like cells within the marrow cavity. Cleanly dissected ("stripped") bones were unresponsive to A23187 and exhibited many dead and dying cells after 48 h in culture with this agent; in contrast, stripped bones were responsive to PTH. All three treatments showed a similar time course of appearance of osteoclasts. The results indicate that all three agents promote osteoclast formation. There are several possible explanations for the differential effects of A23187 and PTH in stripped bones, including the possibility that the cells of the periosteum are required for the action of A23187 but not of PTH.

AB - Morphologic effects of the ionophore A23187 were compared with those of PTH and 1,25(OH)2 vitamin D3 on fetal rat limb bone cultures. All three treatments resulted in loss of bone and the appearance of multinucleate osteoclasts. Only PTH caused proliferation of fibroblast-like cells within the marrow cavity. Cleanly dissected ("stripped") bones were unresponsive to A23187 and exhibited many dead and dying cells after 48 h in culture with this agent; in contrast, stripped bones were responsive to PTH. All three treatments showed a similar time course of appearance of osteoclasts. The results indicate that all three agents promote osteoclast formation. There are several possible explanations for the differential effects of A23187 and PTH in stripped bones, including the possibility that the cells of the periosteum are required for the action of A23187 but not of PTH.

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Ionophore A23187 promotes osteoclast formation in bone organ culture

Abstract

Keywords

ASJC Scopus subject areas

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