IOP and outflow facility are unchanged following acute and chronic intracameral chondroitinase and hyaluronidase in monkeys

W. C. Hubbard, M. Johnson, H. Gong, B. T. Gabelt, B. Tian, J. A. Peterson, R. Sawhney, T. Freddo, P. L. Kaufman

Research output: Contribution to journalArticlepeer-review

Abstract

Purpose. To determine the effect of acute and chronic loss of glycosaminoglycan from the trabecular meshwork on IOP and outflow facility. Methods. Cynomolgus monkeys received intracameral injections of hyaluronidase (Streptomyces, 5 or 50 units, n=2) or chondroitinase ABC (0.05 or 0.25 units, n=2) biweekly for 8 months (4 months for each dose). IOP was measured at 3, 7, 10 and 14 days after each injection. Outflow facility (2-level constant pressure) was determined at 2, 4, 6, and 8 months. Monkeys were sacrificed 6 days after the last injection and glycosaminoglycan histochemistry of the outflow pathways was examined. Acute outflow facilities (30 min or 2 hours after enzyme) were determined in another group of animals (n=4 for each time/enzyme). Results. IOP and outflow facility were unchanged compared to controls (heat inactivated enzyme) at any time in the acute or chronically treated monkeys. Hyaluronic acid staining was absent in the outflow pathways of chronic hyaluronidase treated eyes. Sulfated glycosaminoglycans were decreased but not completely removed by the chronic chondroitinase treatment. Conclusions. Chronic loss of glycomaminoglycans from the trabecular meshwork does not appear to contribute to the IOP elevation and decrease in outflow facility that accompanies open-angle glaucoma. Surprisingly, no increase in outflow facility was found with acute hyaluronidase or chondroitinase treatment.

Original languageEnglish (US)
Pages (from-to)S822
JournalInvestigative Ophthalmology and Visual Science
Volume37
Issue number3
StatePublished - Feb 15 1996

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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