Isoform expression of the voltage-dependent calcium channel α1E

L. M. Marubio, M. Roenfeld, S. Dasgupta, R. J. Miller, L. H. Philipson

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


The α1E voltage-dependent calcium channel has not been clearly identified with a specific neuronal calcium current. To help identify the role of α1E, we examined differential expression of α1E splice variants in mouse brain and cultured cell lines and examined the gene structure of the region encoding the amino terminal. Three splice variants were analyzed by a ribonuclease protection assay, and a fourth variant reported previously in a fetal human α1E sequence was also detected in mouse brain and a pituitary cell line. Whole brain, telencephalon, and olfactory bulb contained predominantly the splice variant corresponding to α1E-I although other known variants could be detected. Neuroendocrine cells in vitro (BTC3 insulinoma cells and AtT-20 pituitary cell lines) expressed predominantly one α1E isoform. The existence of a 5′ exon accounting for the origin of variant 5′ ends reported in different species was suggested by the sequence of the mouse α1E gene in the region encoding the amino terminal.

Original languageEnglish (US)
Pages (from-to)243-251
Number of pages9
JournalReceptors and Channels
Issue number4
StatePublished - 1996


  • Gene
  • Ion channel
  • Isoform
  • Voltage dependent

ASJC Scopus subject areas

  • Pharmacology
  • Endocrinology
  • Clinical Biochemistry
  • Cell Biology


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