Isolation and characterization of microsatellite loci in Penstemon rostriflorus (Plantaginaceae) and cross-species amplification

Andrea T. Kramer; Jeremie B. Fant

doi:10.1111/j.1471-8286.2007.01754.x

Isolation and characterization of microsatellite loci in Penstemon rostriflorus (Plantaginaceae) and cross-species amplification

Andrea T. Kramer, Jeremie B. Fant^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Eight novel microsatellite primer pairs are presented for Penstemon rostriflorus, representing the first microsatellite markers available for this genus. Loci were characterized for 20 individuals from two populations in the Great Basin, USA. All loci are polymorphic within P. rostriflorus (seven to 13 alleles per locus; observed heterozygosity between 0.40 and 0.95), and therefore useful for population genetic studies within the species. Cross-species transferability was tested on 40 additional species of Penstemon, and results indicate that these primers pairs will likely be useful for population genetic studies on many Penstemon species.

Original language	English (US)
Pages (from-to)	998-1001
Number of pages	4
Journal	Molecular Ecology Notes
Volume	7
Issue number	6
DOIs	https://doi.org/10.1111/j.1471-8286.2007.01754.x
State	Published - Nov 2007

Keywords

Great Basin
Microsatellite
Penstemon
Plantaginaceae
Population genetics

ASJC Scopus subject areas

Biochemistry
Ecology
General Biochemistry, Genetics and Molecular Biology

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10.1111/j.1471-8286.2007.01754.x

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title = "Isolation and characterization of microsatellite loci in Penstemon rostriflorus (Plantaginaceae) and cross-species amplification",

abstract = "Eight novel microsatellite primer pairs are presented for Penstemon rostriflorus, representing the first microsatellite markers available for this genus. Loci were characterized for 20 individuals from two populations in the Great Basin, USA. All loci are polymorphic within P. rostriflorus (seven to 13 alleles per locus; observed heterozygosity between 0.40 and 0.95), and therefore useful for population genetic studies within the species. Cross-species transferability was tested on 40 additional species of Penstemon, and results indicate that these primers pairs will likely be useful for population genetic studies on many Penstemon species.",

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AU - Fant, Jeremie B.

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N2 - Eight novel microsatellite primer pairs are presented for Penstemon rostriflorus, representing the first microsatellite markers available for this genus. Loci were characterized for 20 individuals from two populations in the Great Basin, USA. All loci are polymorphic within P. rostriflorus (seven to 13 alleles per locus; observed heterozygosity between 0.40 and 0.95), and therefore useful for population genetic studies within the species. Cross-species transferability was tested on 40 additional species of Penstemon, and results indicate that these primers pairs will likely be useful for population genetic studies on many Penstemon species.

AB - Eight novel microsatellite primer pairs are presented for Penstemon rostriflorus, representing the first microsatellite markers available for this genus. Loci were characterized for 20 individuals from two populations in the Great Basin, USA. All loci are polymorphic within P. rostriflorus (seven to 13 alleles per locus; observed heterozygosity between 0.40 and 0.95), and therefore useful for population genetic studies within the species. Cross-species transferability was tested on 40 additional species of Penstemon, and results indicate that these primers pairs will likely be useful for population genetic studies on many Penstemon species.

KW - Great Basin

KW - Microsatellite

KW - Penstemon

KW - Plantaginaceae

KW - Population genetics

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JO - Molecular Ecology Notes

JF - Molecular Ecology Notes

IS - 6

ER -

Isolation and characterization of microsatellite loci in Penstemon rostriflorus (Plantaginaceae) and cross-species amplification

Abstract

Keywords

ASJC Scopus subject areas

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