Isolation and characterization of microsatellite loci in Penstemon rostriflorus (Plantaginaceae) and cross-species amplification

Andrea T. Kramer, Jeremie B. Fant*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Eight novel microsatellite primer pairs are presented for Penstemon rostriflorus, representing the first microsatellite markers available for this genus. Loci were characterized for 20 individuals from two populations in the Great Basin, USA. All loci are polymorphic within P. rostriflorus (seven to 13 alleles per locus; observed heterozygosity between 0.40 and 0.95), and therefore useful for population genetic studies within the species. Cross-species transferability was tested on 40 additional species of Penstemon, and results indicate that these primers pairs will likely be useful for population genetic studies on many Penstemon species.

Original languageEnglish (US)
Pages (from-to)998-1001
Number of pages4
JournalMolecular Ecology Notes
Volume7
Issue number6
DOIs
StatePublished - Nov 1 2007

Keywords

  • Great Basin
  • Microsatellite
  • Penstemon
  • Plantaginaceae
  • Population genetics

ASJC Scopus subject areas

  • Biochemistry
  • Ecology
  • Biochemistry, Genetics and Molecular Biology(all)

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