Isolation and chromosomal localization of a novel nonerythroid ankyrin gene

W. T. Tse, J. C. Menninger, T. L. Yang-Feng, U. Francke, K. E. Sahr, S. E. Lux, D. C. Ward, B. G. Forget*

*Corresponding author for this work

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

Immunoreactive isoforms of erythrocyte ankyrin have been shown to be present in a variety of nonerythroid tissues. Isolation of the genes that encode these isoforms will clarify their relationship to erythrocyte ankyrin. Using an erythrocyte ankyrin cDNA clone as a hybridization probe, we screened a human genomic library and isolated a clone that hybridizes with the probe at low stringency but not at high stringency. Partial nucleotide sequence of the clone revealed the presence of a 99-bp segment that is homologous to an exon of the erythrocyte ankyrin gene. Northern analysis showed that a labeled fragment of the clone hybridized to a 7-kb message in RNA of fetal brain but not of erythroid cells, suggesting that this clone is part of a novel gere that is expressed predominantly in nonerythroid tissue. Comparison of the sequence of the genomic clone with that of a recently isolated cDNA clone for brain ankyrin (Otto et al., 1989) showed identity of 96 of 99 bp between the putative exon and a segment of the cDNA clone (V. Bennett, personal communication, 1991), suggesting that the genomic clone is part of a gene for nonerythroid ankyrin, which we have designated ANK2. By analysis of somatic cell hybrids and fluorescence in situ hybridization, we assigned ANK2 to human chromosome 4 at a position equivalent to bands 4q25-q27.

Original languageEnglish (US)
Pages (from-to)858-866
Number of pages9
JournalGenomics
Volume10
Issue number4
DOIs
StatePublished - Aug 1991

ASJC Scopus subject areas

  • Genetics

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    Tse, W. T., Menninger, J. C., Yang-Feng, T. L., Francke, U., Sahr, K. E., Lux, S. E., Ward, D. C., & Forget, B. G. (1991). Isolation and chromosomal localization of a novel nonerythroid ankyrin gene. Genomics, 10(4), 858-866. https://doi.org/10.1016/0888-7543(91)90173-C