Isolation and culture of astrocytes from the retina and optic nerve

Thomas J. Lukas*, Ai Ling Wang

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations


Detailed methods for the preparation of optic nerve head (ONH) astrocytes from human donor eyes and retinal astrocytes from rat eyes are described. Included is the immunopanning method used for ONH astrocyte isolation as well as cell characterization. The isolation of purified retinal astrocytes is outlined as a method applicable to rodent and other mammalian retinas.

Original languageEnglish (US)
Title of host publicationAstrocytes
Subtitle of host publicationMethods and Protocols
EditorsRichard Milner
Number of pages11
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Glia
  • Immunopanning
  • Optic nerve head
  • Retina

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology


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