Isolation and culture of astrocytes from the retina and optic nerve

Thomas John Lukas*, Ai Ling Wang

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

Detailed methods for the preparation of optic nerve head (ONH) astrocytes from human donor eyes and retinal astrocytes from rat eyes are described. Included is the immunopanning method used for ONH astrocyte isolation as well as cell characterization. The isolation of purified retinal astrocytes is outlined as a method applicable to rodent and other mammalian retinas.

Original languageEnglish (US)
Title of host publicationAstrocytes
Subtitle of host publicationMethods and Protocols
Pages105-115
Number of pages11
DOIs
StatePublished - Jan 23 2012

Publication series

NameMethods in Molecular Biology
Volume814
ISSN (Print)1064-3745

Keywords

  • Glia
  • Immunopanning
  • Optic nerve head
  • Retina

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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