Abstract
Like corneal endothelial cells, human trabecular meshwork cells are believed to be of neural crest origin, but demonstrate physiological properties and an antithrombogenic surface similar to vascular endothelial cells. One current method for isolating trabecular meshwork cells utilizes the motile nature of these cells to migrate away from a trabecular meshwork explant in culture to more distal regions of the culture dish. This 'outgrowth' technique is limited in practice by the relatively small number of cells that migrate per explant per unit time, thus hindering the ability to gather sufficient numbers of cells for comprehensive experimentation. For this reason, we have modified an extracellular matrix digestion technique in current use for the isolation of microvascular endothelial cells to isolate human trabecular meshwork cells. This procedure is both efficient and rapid for isolating large numbers of trabecular meshwork cells and results in the availability of trabecular meshwork cells in sufficient quantities for subsequent experimentation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 611-617 |
| Number of pages | 7 |
| Journal | Current eye research |
| Volume | 14 |
| Issue number | 7 |
| DOIs | |
| State | Published - 1995 |
| Externally published | Yes |
Funding
This work was supported by NEVNIH Grant #R03 EY09202-1, the Pharmaceutical Research and Manufacturers of America Foundation, the Glaucoma Foundation and the Arizona Lions Eye Bank of Phoenix and the Lions Eye and Tissue Bank of Missouri. We thank Dr James Hoying for his technical assistance and Dr Annelies de Kater for demonstrating the blunt dissection technique.
Keywords
- Cell culture
- Collagenase
- Extracellular matrix
- Trabecular meshwork
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience
- Ophthalmology