Isolation and sequence of a novel human chondrocyte protein related to mammalian members of the chitinase protein family

Bo Hu, Kien Trinh, William F. Figueira, Paul A. Price*

*Corresponding author for this work

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

We describe the isolation of a novel protein from the conditioned medium of human articular cartilage chondrocytes in primary culture. This 39-kDa protein has the N-terminal sequence YKL, which we have termed YKL. 39. The 1434-nucleotide sequence of the YKL-39 cDNA predicts a 385-residue initial translation product and a 364-residue mature YKL-39. The amino acid sequence of YKL-39 is most closely related to YKL-40, followed by macrophage chitotriosidase, oviductal glycoprotein, and macrophage YM-1. All five proteins share significant sequence identity with bacterial chitinases and have the probable structure of an (αβ)8 barrel. YKL-39 lacks the active site glutamate, which is essential for the activity of chitinases, and as expected has no chitinase activity. The highest level of YKL-39 mRNA expression is seen in chondrocytes, followed by synoviocytes, lung, and heart. YKL-39 accounts for 4% of the protein in chondrocyte-conditioned medium, prostromelysin accounts for 17%, and YKL-40 accounts for 33%. In contrast to YKL-40, YKL-39 is not a glycoprotein and does not bind to heparin.

Original languageEnglish (US)
Pages (from-to)19415-19420
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number32
DOIs
StatePublished - Aug 27 1996

Fingerprint

Chitinases
Chondrocytes
Macrophages
Conditioned Culture Medium
Glycoproteins
Proteins
Cartilage
Articular Cartilage
Heparin
Glutamic Acid
Amino Acid Sequence
Catalytic Domain
Nucleotides
Complementary DNA
Amino Acids
Lung
Messenger RNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Hu, Bo ; Trinh, Kien ; Figueira, William F. ; Price, Paul A. / Isolation and sequence of a novel human chondrocyte protein related to mammalian members of the chitinase protein family. In: Journal of Biological Chemistry. 1996 ; Vol. 271, No. 32. pp. 19415-19420.
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Isolation and sequence of a novel human chondrocyte protein related to mammalian members of the chitinase protein family. / Hu, Bo; Trinh, Kien; Figueira, William F.; Price, Paul A.

In: Journal of Biological Chemistry, Vol. 271, No. 32, 27.08.1996, p. 19415-19420.

Research output: Contribution to journalArticle

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