Nucleoli with characteristic morphological abnormalities induced by selected carcinogens and antimetabolites can be isolated from small quantities of rat liver in a form which closely resembles their in situ appearance. Segregation of the nucleolus does not render it more susceptible to fragmentation by sonic isolation. This observation and the related technique not only offer a useful method for ultrastructural study of large numbers of nucleoli in ultrathin sections but also indicate that metabolic studies on isolated nucleoli with experimentally induced morphological abnormalities are reasonably representative of the abnormality as it exists in situ.
ASJC Scopus subject areas
- Cell Biology