Isolation, characterization and chromosomal localization of a human pseudogene for hexokinase II

Hossein Ardehali; Richard L. Printz; Stephen Koch; John A. Phillips; Daryl K. Granner

doi:10.1016/0378-1119(95)00449-G

Isolation, characterization and chromosomal localization of a human pseudogene for hexokinase II

Hossein Ardehali, Richard L. Printz, Stephen Koch, John A. Phillips, Daryl K. Granner^*

^*Corresponding author for this work

Medicine, Cardiology Division

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A processed pseudogene for hexokinase II (HKII), the first such reported for a member of the hexokinase gene family, was isolated from a human genomic library by using a rat HKII cDNA as a probe. The pseudogene contains a region that is identical to the open reading frame of the human HKII cDNA at 97% of the nucleotide positions, but it contains several frameshift mutations, small deletions and insertions, and several stop codons. The human HKII pseudogene is located on the X chromosome and is integrated into a long interspersed nuclear repetitive DNA element (LINE). We estimate that this integration event occurred approximately 14-16 Myr (million years) ago.

Original language	English (US)
Pages (from-to)	357-361
Number of pages	5
Journal	Gene
Volume	164
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(95)00449-G
State	Published - Oct 27 1995

Keywords

LINE element
Somatic cell hybrids
X chromosome
evolution
processed pseudogene

ASJC Scopus subject areas

Genetics

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10.1016/0378-1119(95)00449-G

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title = "Isolation, characterization and chromosomal localization of a human pseudogene for hexokinase II",

abstract = "A processed pseudogene for hexokinase II (HKII), the first such reported for a member of the hexokinase gene family, was isolated from a human genomic library by using a rat HKII cDNA as a probe. The pseudogene contains a region that is identical to the open reading frame of the human HKII cDNA at 97% of the nucleotide positions, but it contains several frameshift mutations, small deletions and insertions, and several stop codons. The human HKII pseudogene is located on the X chromosome and is integrated into a long interspersed nuclear repetitive DNA element (LINE). We estimate that this integration event occurred approximately 14-16 Myr (million years) ago.",

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AU - Ardehali, Hossein

AU - Printz, Richard L.

AU - Koch, Stephen

AU - Phillips, John A.

AU - Granner, Daryl K.

PY - 1995/10/27

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N2 - A processed pseudogene for hexokinase II (HKII), the first such reported for a member of the hexokinase gene family, was isolated from a human genomic library by using a rat HKII cDNA as a probe. The pseudogene contains a region that is identical to the open reading frame of the human HKII cDNA at 97% of the nucleotide positions, but it contains several frameshift mutations, small deletions and insertions, and several stop codons. The human HKII pseudogene is located on the X chromosome and is integrated into a long interspersed nuclear repetitive DNA element (LINE). We estimate that this integration event occurred approximately 14-16 Myr (million years) ago.

AB - A processed pseudogene for hexokinase II (HKII), the first such reported for a member of the hexokinase gene family, was isolated from a human genomic library by using a rat HKII cDNA as a probe. The pseudogene contains a region that is identical to the open reading frame of the human HKII cDNA at 97% of the nucleotide positions, but it contains several frameshift mutations, small deletions and insertions, and several stop codons. The human HKII pseudogene is located on the X chromosome and is integrated into a long interspersed nuclear repetitive DNA element (LINE). We estimate that this integration event occurred approximately 14-16 Myr (million years) ago.

KW - LINE element

KW - Somatic cell hybrids

KW - X chromosome

KW - evolution

KW - processed pseudogene

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Isolation, characterization and chromosomal localization of a human pseudogene for hexokinase II

Abstract

Keywords

ASJC Scopus subject areas

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