TY - JOUR
T1 - Isolation of a gene encoding a novel spectinomycin phosphotransferase from Legionella pneumophila
AU - Suter, Todd M.
AU - Viswanathan, V. K.
AU - Cianciotto, Nicholas P.
PY - 1997/6
Y1 - 1997/6
N2 - A gene capable of conferring spectinomycin resistance was isolated from Legionella pneumophila, the agent of Legionnaires' disease. The gene (aph) encoded a 36-kDa protein which has similarity to aminoglycoside phosphotransferases. Biochemical analysis confirmed that aph encodes a phosphotransferase which modifies spectinomycin but not hygromycin, kanamycin, or streptomycin. The strain that was the source of aph demonstrated resistance to spectinomycin, and Southern hybridizations determined that aph also exists in other legionellae.
AB - A gene capable of conferring spectinomycin resistance was isolated from Legionella pneumophila, the agent of Legionnaires' disease. The gene (aph) encoded a 36-kDa protein which has similarity to aminoglycoside phosphotransferases. Biochemical analysis confirmed that aph encodes a phosphotransferase which modifies spectinomycin but not hygromycin, kanamycin, or streptomycin. The strain that was the source of aph demonstrated resistance to spectinomycin, and Southern hybridizations determined that aph also exists in other legionellae.
UR - http://www.scopus.com/inward/record.url?scp=0030983278&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030983278&partnerID=8YFLogxK
U2 - 10.1128/aac.41.6.1385
DO - 10.1128/aac.41.6.1385
M3 - Article
C2 - 9174205
AN - SCOPUS:0030983278
SN - 0066-4804
VL - 41
SP - 1385
EP - 1388
JO - Antimicrobial agents and chemotherapy
JF - Antimicrobial agents and chemotherapy
IS - 6
ER -
