Isolation of a gene encoding a novel spectinomycin phosphotransferase from Legionella pneumophila

Todd M. Suter, V. K. Viswanathan, Nicholas P. Cianciotto*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

A gene capable of conferring spectinomycin resistance was isolated from Legionella pneumophila, the agent of Legionnaires' disease. The gene (aph) encoded a 36-kDa protein which has similarity to aminoglycoside phosphotransferases. Biochemical analysis confirmed that aph encodes a phosphotransferase which modifies spectinomycin but not hygromycin, kanamycin, or streptomycin. The strain that was the source of aph demonstrated resistance to spectinomycin, and Southern hybridizations determined that aph also exists in other legionellae.

Original languageEnglish (US)
Pages (from-to)1385-1388
Number of pages4
JournalAntimicrobial agents and chemotherapy
Volume41
Issue number6
DOIs
StatePublished - Jun 1997

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

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