Inclusion bodies (IB) synthesized during sporulation and enterotoxin formation by Clostridium perfringens were isolated. Sporulating cells were lysed by sonication in the presence of protease inhibitors. IB were isolated by centrifugation in linear gradients of sucrose, sodium bromide or sodium diatrizoate and banded at buoyant densities of 1.33-1.36 g/cm3, 1.30-1.34 g/cm3 and 1.33 g/cm3, respectively. Isolated IB were treated with detergent to remove attached cell membrane. They ranged in size from 0.5-1.4 μm long and from 0.2-0.5 μm wide. They were found to be serologically related to purified enterotoxin.
ASJC Scopus subject areas
- Molecular Biology