Isolation of an inclusion body from sporulating, enterotoxin-positive Clostridium perfringens

Alicia Loffler, Ronald G. Labbé

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Inclusion bodies (IB) synthesized during sporulation and enterotoxin formation by Clostridium perfringens were isolated. Sporulating cells were lysed by sonication in the presence of protease inhibitors. IB were isolated by centrifugation in linear gradients of sucrose, sodium bromide or sodium diatrizoate and banded at buoyant densities of 1.33-1.36 g/cm3, 1.30-1.34 g/cm3 and 1.33 g/cm3, respectively. Isolated IB were treated with detergent to remove attached cell membrane. They ranged in size from 0.5-1.4 μm long and from 0.2-0.5 μm wide. They were found to be serologically related to purified enterotoxin.

Original languageEnglish (US)
Pages (from-to)143-147
Number of pages5
JournalFEMS Microbiology Letters
Volume27
Issue number2
DOIs
StatePublished - Jan 1 1985

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

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