Isolation of an inclusion body from sporulating, enterotoxin-positive Clostridium perfringens

Alicia Loffler; Ronald G. Labbé

doi:10.1111/j.1574-6968.1985.tb00657.x

Isolation of an inclusion body from sporulating, enterotoxin-positive Clostridium perfringens

Alicia Loffler, Ronald G. Labbé^*

^*Corresponding author for this work

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Abstract

Inclusion bodies (IB) synthesized during sporulation and enterotoxin formation by Clostridium perfringens were isolated. Sporulating cells were lysed by sonication in the presence of protease inhibitors. IB were isolated by centrifugation in linear gradients of sucrose, sodium bromide or sodium diatrizoate and banded at buoyant densities of 1.33-1.36 g/cm³, 1.30-1.34 g/cm³ and 1.33 g/cm³, respectively. Isolated IB were treated with detergent to remove attached cell membrane. They ranged in size from 0.5-1.4 μm long and from 0.2-0.5 μm wide. They were found to be serologically related to purified enterotoxin.

Original language	English (US)
Pages (from-to)	143-147
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	27
Issue number	2
DOIs	https://doi.org/10.1111/j.1574-6968.1985.tb00657.x
State	Published - May 1985

Keywords

Density gradients
immunodiffusion
spores

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1111/j.1574-6968.1985.tb00657.x

Cite this

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title = "Isolation of an inclusion body from sporulating, enterotoxin-positive Clostridium perfringens",

abstract = "Inclusion bodies (IB) synthesized during sporulation and enterotoxin formation by Clostridium perfringens were isolated. Sporulating cells were lysed by sonication in the presence of protease inhibitors. IB were isolated by centrifugation in linear gradients of sucrose, sodium bromide or sodium diatrizoate and banded at buoyant densities of 1.33-1.36 g/cm3, 1.30-1.34 g/cm3 and 1.33 g/cm3, respectively. Isolated IB were treated with detergent to remove attached cell membrane. They ranged in size from 0.5-1.4 μm long and from 0.2-0.5 μm wide. They were found to be serologically related to purified enterotoxin.",

keywords = "Density gradients, immunodiffusion, spores",

author = "Alicia Loffler and Labb{\'e}, {Ronald G.}",

note = "Funding Information: This work was supported by U.S.D.A. Department of Agriculture Experiment Station, Project No. 484. We thank Dr. R. Rufner for assistance with the electron microscope.",

year = "1985",

month = may,

doi = "10.1111/j.1574-6968.1985.tb00657.x",

language = "English (US)",

volume = "27",

pages = "143--147",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Wiley-Blackwell",

number = "2",

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T1 - Isolation of an inclusion body from sporulating, enterotoxin-positive Clostridium perfringens

AU - Loffler, Alicia

AU - Labbé, Ronald G.

N1 - Funding Information: This work was supported by U.S.D.A. Department of Agriculture Experiment Station, Project No. 484. We thank Dr. R. Rufner for assistance with the electron microscope.

PY - 1985/5

Y1 - 1985/5

N2 - Inclusion bodies (IB) synthesized during sporulation and enterotoxin formation by Clostridium perfringens were isolated. Sporulating cells were lysed by sonication in the presence of protease inhibitors. IB were isolated by centrifugation in linear gradients of sucrose, sodium bromide or sodium diatrizoate and banded at buoyant densities of 1.33-1.36 g/cm3, 1.30-1.34 g/cm3 and 1.33 g/cm3, respectively. Isolated IB were treated with detergent to remove attached cell membrane. They ranged in size from 0.5-1.4 μm long and from 0.2-0.5 μm wide. They were found to be serologically related to purified enterotoxin.

AB - Inclusion bodies (IB) synthesized during sporulation and enterotoxin formation by Clostridium perfringens were isolated. Sporulating cells were lysed by sonication in the presence of protease inhibitors. IB were isolated by centrifugation in linear gradients of sucrose, sodium bromide or sodium diatrizoate and banded at buoyant densities of 1.33-1.36 g/cm3, 1.30-1.34 g/cm3 and 1.33 g/cm3, respectively. Isolated IB were treated with detergent to remove attached cell membrane. They ranged in size from 0.5-1.4 μm long and from 0.2-0.5 μm wide. They were found to be serologically related to purified enterotoxin.

KW - Density gradients

KW - immunodiffusion

KW - spores

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SN - 0378-1097

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ER -

Isolation of an inclusion body from sporulating, enterotoxin-positive Clostridium perfringens

Abstract

Keywords

ASJC Scopus subject areas

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