Isolation of band-specific (CA)n microsatellites from human chromosome 8q24.1

Lei Xu; Jiahui Xia; Qian Pan; Tao Cai; Hanxiang Deng

Isolation of band-specific (CA)_n microsatellites from human chromosome 8q24.1

Lei Xu^*, Jiahui Xia, Qian Pan, Tao Cai, Hanxiang Deng

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

Abstract

Fourty eight recombinant plasmids containing CA repeats were isolated from human chromosome 8q24.1 band-specific pUC19 library. Among them, inserted DNA fragments from 12 plasmids were sequenced, and one new highly polymorphic (CA)_n microsatellite was obtained (coding: D8S7F). It contained 11 alleles. The estimated heterozygosity reached 0.84 and 0.83 among Chinese Han Nationality people and American Anglo-Saxons respectively. It was assigned to chromosome 8 by using a human-rodent hybrid cell panel.

Original language	English (US)
Pages (from-to)	4-6
Number of pages	3
Journal	Acta Genetica Sinica
Volume	24
Issue number	1
State	Published - Dec 1 1997

Keywords

(CA)
Chromosome band-specific
Microsatellite DNA
pUC19 library

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

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title = "Isolation of band-specific (CA)n microsatellites from human chromosome 8q24.1",

abstract = "Fourty eight recombinant plasmids containing CA repeats were isolated from human chromosome 8q24.1 band-specific pUC19 library. Among them, inserted DNA fragments from 12 plasmids were sequenced, and one new highly polymorphic (CA)n microsatellite was obtained (coding: D8S7F). It contained 11 alleles. The estimated heterozygosity reached 0.84 and 0.83 among Chinese Han Nationality people and American Anglo-Saxons respectively. It was assigned to chromosome 8 by using a human-rodent hybrid cell panel.",

keywords = "(CA), Chromosome band-specific, Microsatellite DNA, pUC19 library",

author = "Lei Xu and Jiahui Xia and Qian Pan and Tao Cai and Hanxiang Deng",

year = "1997",

month = dec,

day = "1",

language = "English (US)",

volume = "24",

pages = "4--6",

journal = "Acta Genetica Sinica",

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TY - JOUR

T1 - Isolation of band-specific (CA)n microsatellites from human chromosome 8q24.1

AU - Xu, Lei

AU - Xia, Jiahui

AU - Pan, Qian

AU - Cai, Tao

AU - Deng, Hanxiang

PY - 1997/12/1

Y1 - 1997/12/1

N2 - Fourty eight recombinant plasmids containing CA repeats were isolated from human chromosome 8q24.1 band-specific pUC19 library. Among them, inserted DNA fragments from 12 plasmids were sequenced, and one new highly polymorphic (CA)n microsatellite was obtained (coding: D8S7F). It contained 11 alleles. The estimated heterozygosity reached 0.84 and 0.83 among Chinese Han Nationality people and American Anglo-Saxons respectively. It was assigned to chromosome 8 by using a human-rodent hybrid cell panel.

AB - Fourty eight recombinant plasmids containing CA repeats were isolated from human chromosome 8q24.1 band-specific pUC19 library. Among them, inserted DNA fragments from 12 plasmids were sequenced, and one new highly polymorphic (CA)n microsatellite was obtained (coding: D8S7F). It contained 11 alleles. The estimated heterozygosity reached 0.84 and 0.83 among Chinese Han Nationality people and American Anglo-Saxons respectively. It was assigned to chromosome 8 by using a human-rodent hybrid cell panel.

KW - (CA)

KW - Chromosome band-specific

KW - Microsatellite DNA

KW - pUC19 library

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M3 - Article

C2 - 9167365

AN - SCOPUS:0030629474

SN - 0379-4172

VL - 24

SP - 4

EP - 6

JO - Acta Genetica Sinica

JF - Acta Genetica Sinica

IS - 1

ER -

Isolation of band-specific (CA)_n microsatellites from human chromosome 8q24.1

Abstract

Keywords

ASJC Scopus subject areas

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