Isolation of cochlear inner hair cells

David Z Z He, Jing Zheng, Roxanne Edge, Peter Dallos

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

In order to identify hair cell specific genes, it is essential to obtain isolated hair cells in quantity. While whole-cell recordings have been made from isolated inner hair cells (IHCs) from guinea pigs, detailed methods for obtaining a fairly large amount of isolated inner hair cells have not been published. Here we describe a protocol that can yield a fairly large amount of isolated gerbil IHCs. This technique can provide sufficient numbers of solitary IHCs for either electrophysiological studies of the cell's membrane properties or identifying genes related to IHC functions using techniques of molecular biology. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)156-160
Number of pages5
JournalHearing Research
Volume145
Issue number1-2
DOIs
StatePublished - Jun 27 2000

Fingerprint

Inner Auditory Hair Cells
Gerbillinae
Patch-Clamp Techniques
Genes
Molecular Biology
Guinea Pigs
Cell Membrane

Keywords

  • Gerbil
  • Inner hair cell
  • Isolation technique
  • Morphology
  • Outer hair cell
  • Voltage-clamp

ASJC Scopus subject areas

  • Sensory Systems

Cite this

He, David Z Z ; Zheng, Jing ; Edge, Roxanne ; Dallos, Peter. / Isolation of cochlear inner hair cells. In: Hearing Research. 2000 ; Vol. 145, No. 1-2. pp. 156-160.
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He, DZZ, Zheng, J, Edge, R & Dallos, P 2000, 'Isolation of cochlear inner hair cells', Hearing Research, vol. 145, no. 1-2, pp. 156-160. https://doi.org/10.1016/S0378-5955(00)00084-8

Isolation of cochlear inner hair cells. / He, David Z Z; Zheng, Jing; Edge, Roxanne; Dallos, Peter.

In: Hearing Research, Vol. 145, No. 1-2, 27.06.2000, p. 156-160.

Research output: Contribution to journalArticle

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AU - Zheng, Jing

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AU - Dallos, Peter

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