Isolation of cochlear inner hair cells

David Z.Z. He; Jing Zheng; Roxanne Edge; Peter Dallos

doi:10.1016/S0378-5955(00)00084-8

Isolation of cochlear inner hair cells

David Z.Z. He^*, Jing Zheng, Roxanne Edge, Peter Dallos

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

In order to identify hair cell specific genes, it is essential to obtain isolated hair cells in quantity. While whole-cell recordings have been made from isolated inner hair cells (IHCs) from guinea pigs, detailed methods for obtaining a fairly large amount of isolated inner hair cells have not been published. Here we describe a protocol that can yield a fairly large amount of isolated gerbil IHCs. This technique can provide sufficient numbers of solitary IHCs for either electrophysiological studies of the cell's membrane properties or identifying genes related to IHC functions using techniques of molecular biology. Copyright (C) 2000 Elsevier Science B.V.

Original language	English (US)
Pages (from-to)	156-160
Number of pages	5
Journal	Hearing research
Volume	145
Issue number	1-2
DOIs	https://doi.org/10.1016/S0378-5955(00)00084-8
State	Published - 2000

Funding

The research was supported by HIH Grant DC000708 from NIDCD to P.D. and by a grant from the Deafness Research Foundation to D.H.

Keywords

Gerbil
Inner hair cell
Isolation technique
Morphology
Outer hair cell
Voltage-clamp

ASJC Scopus subject areas

Sensory Systems

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10.1016/S0378-5955(00)00084-8

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title = "Isolation of cochlear inner hair cells",

abstract = "In order to identify hair cell specific genes, it is essential to obtain isolated hair cells in quantity. While whole-cell recordings have been made from isolated inner hair cells (IHCs) from guinea pigs, detailed methods for obtaining a fairly large amount of isolated inner hair cells have not been published. Here we describe a protocol that can yield a fairly large amount of isolated gerbil IHCs. This technique can provide sufficient numbers of solitary IHCs for either electrophysiological studies of the cell's membrane properties or identifying genes related to IHC functions using techniques of molecular biology. Copyright (C) 2000 Elsevier Science B.V.",

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author = "He, {David Z.Z.} and Jing Zheng and Roxanne Edge and Peter Dallos",

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T1 - Isolation of cochlear inner hair cells

AU - He, David Z.Z.

AU - Zheng, Jing

AU - Edge, Roxanne

AU - Dallos, Peter

N1 - Funding Information: The research was supported by HIH Grant DC000708 from NIDCD to P.D. and by a grant from the Deafness Research Foundation to D.H.

PY - 2000

Y1 - 2000

N2 - In order to identify hair cell specific genes, it is essential to obtain isolated hair cells in quantity. While whole-cell recordings have been made from isolated inner hair cells (IHCs) from guinea pigs, detailed methods for obtaining a fairly large amount of isolated inner hair cells have not been published. Here we describe a protocol that can yield a fairly large amount of isolated gerbil IHCs. This technique can provide sufficient numbers of solitary IHCs for either electrophysiological studies of the cell's membrane properties or identifying genes related to IHC functions using techniques of molecular biology. Copyright (C) 2000 Elsevier Science B.V.

AB - In order to identify hair cell specific genes, it is essential to obtain isolated hair cells in quantity. While whole-cell recordings have been made from isolated inner hair cells (IHCs) from guinea pigs, detailed methods for obtaining a fairly large amount of isolated inner hair cells have not been published. Here we describe a protocol that can yield a fairly large amount of isolated gerbil IHCs. This technique can provide sufficient numbers of solitary IHCs for either electrophysiological studies of the cell's membrane properties or identifying genes related to IHC functions using techniques of molecular biology. Copyright (C) 2000 Elsevier Science B.V.

KW - Gerbil

KW - Inner hair cell

KW - Isolation technique

KW - Morphology

KW - Outer hair cell

KW - Voltage-clamp

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JO - Hearing research

JF - Hearing research

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ER -

Isolation of cochlear inner hair cells

Abstract

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Keywords

ASJC Scopus subject areas

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