This chapter considers the approaches available for the isolation of developmentally regulated genes from Dictyostelium, and some of the advantages and drawbacks of each are discussed. The chapter discusses various techniques for the isolation of genes having a particular pattern of regulation, is limited only by imagination. Using these techniques, it is possible to identify genes induced by heat shock, by starvation, expressed at a particular time in the cell cycle, in a particular cell type, or any other fashion that allows the investigator to identify a set of conditions where the genes of interest are expressed and another where they are not. The use of developmental mutants in such gene selection schemes may increase the kinds of schemes that can be devised. For example, it may be possible to use null mutants and subtractive hybridization to isolate a wild-type copy of the mutant gene. The availability of cloned developmentally regulated genes has facilitated investigation of the mechanisms by which the developmental program is regulated, and has given the significant insight into the molecular details of that regulation.
ASJC Scopus subject areas
- Cell Biology