Isolation of differentially expressed cDNAs from p53-dependent apoptotic cells: Activation of the human homologue of the Drosophila peroxidasin gene

Nobuo Horikoshi*, Jainping Cong, Nikoli Kley, Thomas Shenk

*Corresponding author for this work

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Inactivation of the p53 tumor suppressor protein has been observed in a large number of human cancers. Overexpression of p53 induces either growth arrest or programmed cell death (apoptosis). The growth arrest function of p53 is mediated by induction of p21 (WAF1/CIP1), but the mechanisms underlying p53-dependent apoptosis are still largely unknown. To investigate these mechanisms, we have identified six differentially expressed transcripts in a human colon cancer cell line undergoing p53 dependent apoptosis. One of the p53-responsive genes showed significant homology to Drosophila peroxidasin, an extracellular matrix-associated peroxidase, and is likely to be its human homologue. Our results suggest a possible connection between p53-dependent apoptosis and the production of reactive oxygen species.

Original languageEnglish (US)
Pages (from-to)864-869
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume261
Issue number3
DOIs
StatePublished - Aug 11 1999

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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