Isolation of the target antigen of human anti-tubular basement membrane antibody-associated interstitial nephritis

M. D. Clayman, L. Michaud, J. Brentjens, G. A. Andres, N. A. Kefalides, E. G. Neilson

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

Using a monoclonal anti-tubular basement membrane antibody (αTBM-Ab) affinity column, we isolated from collagenase-solubilized human renal tissue (HSRTA) a predominantly 48,000-mol-wt moiety (H3M-1) which is selectively recognized by antisera from two patients with αTBM-Ab-associated interstitial nephritis (αTBM disease). Whereas both antisera had αTBM-Ab titers of 1:64-1:128 by immunofluorescence on tissue sections, their reactivity with H3M-1 in a solid-phase radioimmunoassay was demonstrable at dilutions up to 1:10,000. While these sera displayed some reactivity with pre-column HSRTA, this was markedly less than with H3M-1. HSRTA depleted of H3M-1 by passage over the αTBM-Ab affinity column was almost completely depleted of reactivity. Neither pooled normal human sera nor sera from patients with a variety of renal lesions not associated with αTBM-Ab (including interstitial nephritis and antiglomerular basement membrane disease) were reactive with H3M-1. Both patient antisera containing αTBM-Ab were also highly reactive with R3M-1, the 48,000-mol-wt rabbit glycoprotein antigen of experimental αTBM disease. Furthermore, a competitive inhibition radioimmunoassay revealed that αTBM-Ab from rodents with experimental αTBM disease could inhibit 45-98% of the R3M-1 binding reactivity of patient antisera and 85% of the H3M-1 binding reactivity of patient antisera, thus suggesting paratypic cross-reactivity. We conclude, therefore, that tubular basement membrane target epitopes and their paratypic recognition are highly conserved among mammals.

Original languageEnglish (US)
Pages (from-to)1143-1147
Number of pages5
JournalJournal of Clinical Investigation
Volume77
Issue number4
DOIs
StatePublished - 1986

ASJC Scopus subject areas

  • Medicine(all)

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