Isometric multimodal photoacoustic microscopy based on optically transparent micro-ring ultrasonic detection

Biqin Dong, Hao Li, Zhen Zhang, Kevin Zhang, Siyu Chen, Cheng Sun, Hao F. Zhang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

50 Scopus citations

Abstract

Photoacoustic microscopy (PAM) is an attractive imaging tool that complements established optical microscopic modalities by providing additional molecular specificities through imaging optical absorption contrast. While the development of optical resolution photoacoustic microscopy (ORPAM) offers high lateral resolution, the acoustically determined axial resolution is limited due to the constraint in ultrasonic detection bandwidth. ORPAM with isometric spatial resolution along both axial and lateral directions is yet to be developed. Although recently developed sophisticated optical illumination and reconstruction methods offer improved axial resolution in ORPAM, the image acquisition procedures are rather complicated, limiting their capabilities for high-speed imaging and being easily integrated with established optical microscopic modalities. Here we report an isometric ORPAM based on an optically transparent micro-ring resonator ultrasonic detector and a commercial inverted microscope platform. Owing to the superior spatial resolution and the ease of integrating our ORPAM with established microscopic modalities, single-cell imaging with extrinsic fluorescence staining, intrinsic autofluorescence, and optical absorption can be achieved simultaneously. This technique holds promise to greatly improve the accessibility of PAM to broader biomedical researchers.

Original languageEnglish (US)
Pages (from-to)169-176
Number of pages8
JournalOptica
Volume2
Issue number2
DOIs
StatePublished - 2015

Keywords

  • All-optical devices
  • Medical and biological imaging
  • Photoacoustic imaging
  • Three-dimensional microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

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