Isoproterenol improves ability of lung to clear edema in rats exposed to hyperoxia

F. J. Saldías, A. Comellas, K. M. Ridge, E. Lecuona, J. I. Sznajder*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

Exposure of adult rats to 100% O2 results in lung injury and decreases active sodium transport and lung edema clearance. It has been reported that β-adrenergic agonists increase lung edema clearance in normal rat lungs by upregulating alveolar epithelial Na+-K+-ATPase function. This study was designed to examine whether isoproterenol (Iso) affects lung edema clearance in rats exposed to 100% O2 for 64 h. Active Na+ transport and lung edema clearance decreased by ~44% in rats exposed to acute hyperoxia. Iso (10-6 M) increased the ability of the lung to clear edema in room-air-breathing rats (from 0.50 ± 0.02 to 0.99 ± 0.05 ml/h) and in rats exposed to 100% O2 (from 0.28 ± 0.03 to 0.86 ± 0.09 ml/h; P <0.001). Disruption of intracellular microtubular transport of ion-transporting proteins by colchicine (0.25 mg/100 g body wt) inhibited the stimulatory effects of Iso in hyperoxia-injured rat lungs, whereas the isomer β-lumicolchicine, which does not affect microtubular transport, did not inhibit active Na+ transport stimulated by Iso. Accordingly, Iso restored the lung's ability to clear edema after hyperoxic lung injury, probably by stimulation of the recruitment of ion-transporting proteins (Na+-K+-ATPase) from intracellular pools to the plasma membrane in rat alveolar epithelium.

Original languageEnglish (US)
Pages (from-to)30-35
Number of pages6
JournalJournal of applied physiology
Volume87
Issue number1
DOIs
StatePublished - Jul 1999

Keywords

  • β-adrenergic
  • Active sodium transport
  • Oxidant lung injury
  • Sodium-potassium- adenosinetriphosphatase

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

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