Abstract
The past decade has revolutionized our understanding of regulatory noncoding RNAs (ncRNAs). Among the most recently identified ncRNAs are downstream-of-gene (DoG)-containing transcripts that are produced by widespread transcriptional readthrough. The discovery of DoGs has set the stage for future studies to address many unanswered questions regarding the mechanisms that promote readthrough transcription, RNA processing, and the cellular functions of the unique transcripts. In this review, we summarize current findings regarding the biogenesis, function, and mechanisms regulating this exciting new class of RNA molecules.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1981-1991 |
| Number of pages | 11 |
| Journal | Molecular cell |
| Volume | 82 |
| Issue number | 11 |
| DOIs | |
| State | Published - Jun 2 2022 |
Funding
We thank Pedro Antonio Avila Lopez and Brianna Monroe for contributing to figure preparation for this review article. We apologize for any oversights or omissions. Research in the Lauberth lab is supported by a grant from the NIH / National Institute of General Medical Sciences ( R35 GM128900 ) to S.M.L.; research in the Shilatifard laboratory is supported by NIH ( R35CA197569 and U54CA231638 ) to A.S.; and research in the Shiekhattar laboratory is supported by funding from the University of Miami Miller School of Medicine, Sylvester Comprehensive Cancer Center and grants R01 GM078455, R01 GM105754 , P30 CA240139 , and DP1 CA228041 from the National Institute of Health to R.S.
Keywords
- CRISPR
- elongation
- gene expression
- next-generation sequencing analysis
- noncoding RNAs
- readthrough transcription
- termination
- transcriptional regulation
- transcriptomics
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology