It's clearly the heart! Optical transparency, cardiac tissue imaging, and computer modelling

Gregory B. Sands; Jesse L. Ashton; Mark L. Trew; David Baddeley; Richard D. Walton; David Benoist; Igor R. Efimov; Nicolas P. Smith; Olivier Bernus; Bruce H. Smaill

doi:10.1016/j.pbiomolbio.2021.06.005

It's clearly the heart! Optical transparency, cardiac tissue imaging, and computer modelling

Gregory B. Sands^*, Jesse L. Ashton, Mark L. Trew, David Baddeley, Richard D. Walton, David Benoist, Igor R. Efimov, Nicolas P. Smith, Olivier Bernus, Bruce H. Smaill

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Recent developments in clearing and microscopy enable 3D imaging with cellular resolution up to the whole organ level. These methods have been used extensively in neurobiology, but their uptake in other fields has been much more limited. Application of this approach to the human heart and effective use of the data acquired present challenges of scale and complexity. Four interlinked issues need to be addressed: 1) efficient clearing and labelling of heart tissue, 2) fast microscopic imaging of human-scale samples, 3) handling and processing of multi-terabyte 3D images, and 4) extraction of structural information in computationally tractable structure-based models of cardiac function. Preliminary studies show that each of these requirements can be achieved with the appropriate application and development of existing technologies.

Original language	English (US)
Pages (from-to)	18-32
Number of pages	15
Journal	Progress in Biophysics and Molecular Biology
Volume	168
DOIs	https://doi.org/10.1016/j.pbiomolbio.2021.06.005
State	Published - Jan 2022

Funding

This work was supported by a grant from the Leducq Foundation and by a Marsden grant ( UOA1620 ) from the Royal Society of New Zealand .

Keywords

Cardiac tissue
Extended-volume imaging
Image processing
Microscopy
Network modelling
Tissue clearing

ASJC Scopus subject areas

Molecular Biology
Biophysics

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10.1016/j.pbiomolbio.2021.06.005

Cite this

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title = "It's clearly the heart! Optical transparency, cardiac tissue imaging, and computer modelling",

abstract = "Recent developments in clearing and microscopy enable 3D imaging with cellular resolution up to the whole organ level. These methods have been used extensively in neurobiology, but their uptake in other fields has been much more limited. Application of this approach to the human heart and effective use of the data acquired present challenges of scale and complexity. Four interlinked issues need to be addressed: 1) efficient clearing and labelling of heart tissue, 2) fast microscopic imaging of human-scale samples, 3) handling and processing of multi-terabyte 3D images, and 4) extraction of structural information in computationally tractable structure-based models of cardiac function. Preliminary studies show that each of these requirements can be achieved with the appropriate application and development of existing technologies.",

keywords = "Cardiac tissue, Extended-volume imaging, Image processing, Microscopy, Network modelling, Tissue clearing",

author = "Sands, {Gregory B.} and Ashton, {Jesse L.} and Trew, {Mark L.} and David Baddeley and Walton, {Richard D.} and David Benoist and Efimov, {Igor R.} and Smith, {Nicolas P.} and Olivier Bernus and Smaill, {Bruce H.}",

note = "Funding Information: This work was supported by a grant from the Leducq Foundation and by a Marsden grant ( UOA1620 ) from the Royal Society of New Zealand . Publisher Copyright: {\textcopyright} 2021 Elsevier Ltd",

year = "2022",

month = jan,

doi = "10.1016/j.pbiomolbio.2021.06.005",

language = "English (US)",

volume = "168",

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journal = "Progress in Biophysics and Molecular Biology",

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AU - Sands, Gregory B.

AU - Ashton, Jesse L.

AU - Trew, Mark L.

AU - Baddeley, David

AU - Walton, Richard D.

AU - Benoist, David

AU - Efimov, Igor R.

AU - Smith, Nicolas P.

AU - Bernus, Olivier

AU - Smaill, Bruce H.

N1 - Funding Information: This work was supported by a grant from the Leducq Foundation and by a Marsden grant ( UOA1620 ) from the Royal Society of New Zealand . Publisher Copyright: © 2021 Elsevier Ltd

PY - 2022/1

Y1 - 2022/1

N2 - Recent developments in clearing and microscopy enable 3D imaging with cellular resolution up to the whole organ level. These methods have been used extensively in neurobiology, but their uptake in other fields has been much more limited. Application of this approach to the human heart and effective use of the data acquired present challenges of scale and complexity. Four interlinked issues need to be addressed: 1) efficient clearing and labelling of heart tissue, 2) fast microscopic imaging of human-scale samples, 3) handling and processing of multi-terabyte 3D images, and 4) extraction of structural information in computationally tractable structure-based models of cardiac function. Preliminary studies show that each of these requirements can be achieved with the appropriate application and development of existing technologies.

AB - Recent developments in clearing and microscopy enable 3D imaging with cellular resolution up to the whole organ level. These methods have been used extensively in neurobiology, but their uptake in other fields has been much more limited. Application of this approach to the human heart and effective use of the data acquired present challenges of scale and complexity. Four interlinked issues need to be addressed: 1) efficient clearing and labelling of heart tissue, 2) fast microscopic imaging of human-scale samples, 3) handling and processing of multi-terabyte 3D images, and 4) extraction of structural information in computationally tractable structure-based models of cardiac function. Preliminary studies show that each of these requirements can be achieved with the appropriate application and development of existing technologies.

KW - Cardiac tissue

KW - Extended-volume imaging

KW - Image processing

KW - Microscopy

KW - Network modelling

KW - Tissue clearing

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It's clearly the heart! Optical transparency, cardiac tissue imaging, and computer modelling

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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