Abstract
Recent developments in clearing and microscopy enable 3D imaging with cellular resolution up to the whole organ level. These methods have been used extensively in neurobiology, but their uptake in other fields has been much more limited. Application of this approach to the human heart and effective use of the data acquired present challenges of scale and complexity. Four interlinked issues need to be addressed: 1) efficient clearing and labelling of heart tissue, 2) fast microscopic imaging of human-scale samples, 3) handling and processing of multi-terabyte 3D images, and 4) extraction of structural information in computationally tractable structure-based models of cardiac function. Preliminary studies show that each of these requirements can be achieved with the appropriate application and development of existing technologies.
Original language | English (US) |
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Pages (from-to) | 18-32 |
Number of pages | 15 |
Journal | Progress in Biophysics and Molecular Biology |
Volume | 168 |
DOIs | |
State | Published - Jan 2022 |
Funding
This work was supported by a grant from the Leducq Foundation and by a Marsden grant ( UOA1620 ) from the Royal Society of New Zealand .
Keywords
- Cardiac tissue
- Extended-volume imaging
- Image processing
- Microscopy
- Network modelling
- Tissue clearing
ASJC Scopus subject areas
- Molecular Biology
- Biophysics