Joint profiling of DNA methylation and chromatin architecture in single cells

Guoqiang Li; Yaping Liu; Yanxiao Zhang; Naoki Kubo; Miao Yu; Rongxin Fang; Manolis Kellis; Bing Ren

doi:10.1038/s41592-019-0502-z

Joint profiling of DNA methylation and chromatin architecture in single cells

Guoqiang Li, Yaping Liu, Yanxiao Zhang, Naoki Kubo, Miao Yu, Rongxin Fang, Manolis Kellis, Bing Ren^*

^*Corresponding author for this work

Biochemistry and Molecular Genetics

Research output: Contribution to journal › Article › peer-review

147 Scopus citations

Abstract

We report a molecular assay, Methyl-HiC, that can simultaneously capture the chromosome conformation and DNA methylome in a cell. Methyl-HiC reveals coordinated DNA methylation status between distal genomic segments that are in spatial proximity in the nucleus, and delineates heterogeneity of both the chromatin architecture and DNA methylome in a mixed population. It enables simultaneous characterization of cell-type-specific chromatin organization and epigenome in complex tissues.

Original language	English (US)
Pages (from-to)	991-993
Number of pages	3
Journal	Nature Methods
Volume	16
Issue number	10
DOIs	https://doi.org/10.1038/s41592-019-0502-z
State	Published - Oct 1 2019

Funding

We thank S. Kuan and B. Li for processing of sequencing. The study was supported in part by the NIH 4D Nucleome program (grant no. U54DK107977) (to B.R.), grant no. 1U01HG007610-01 (to M.K.) and a start-up grant from CCHMC (to Y.L.).

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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AU - Fang, Rongxin

AU - Kellis, Manolis

AU - Ren, Bing

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Joint profiling of DNA methylation and chromatin architecture in single cells

Abstract

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