KAP-1, a novel corepressor for the highly conserved KRAB repression domain

Josh R. Friedman, William J. Fredericks, David E. Jensen, David W. Speicher, Xiao Pei Huang, Eric G. Neilson, Frank J. Rauscher*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

486 Scopus citations

Abstract

The KRAB repression domain is one of the most widely distributed transcriptional effector domains yet identified, but its mechanism of repression is unknown. We have cloned a corepressor, KAP-1, which associates with the KRAB domain but not with KRAB mutants that have lost repression activity. KAP-1 can enhance KRAB-mediated repression and is a repressor when directly tethered to DNA. KAP-1 contains a RING finger, B boxes, and a PHD finger; the RING-B1-B2 structure is required for KRAB binding and corepression. We propose that KAP-1 may be a universal corepressor for the large family of KRAB domain-containing transcription factors.

Original languageEnglish (US)
Pages (from-to)2067-2078
Number of pages12
JournalGenes and Development
Volume10
Issue number16
DOIs
StatePublished - 1996

Keywords

  • Corepressor
  • KRAB domain
  • RING finger
  • zinc-finger

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Fingerprint Dive into the research topics of 'KAP-1, a novel corepressor for the highly conserved KRAB repression domain'. Together they form a unique fingerprint.

Cite this