KDM3B is the H3K9 demethylase involved in transcriptional activation of lmo2 in leukemia

Ji Young Kim, Kee Beom Kim, Gwang Hyeon Eom, Nakwon Choe, Hae Jin Kee, Hye Ju Son, Si Taek Oh, Dong Wook Kim, Jhang Ho Pak, Hee Jo Baek, Hoon Kook, Yoonsoo Hahn, Hyun Kook, Debabrata Chakravarti, Sang Beom Seo*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

Histone lysine methylation and demethylation are considered critical steps in transcriptional regulation. In this report, we performed chromatin immunoprecipitation with microarray technology (ChIP-chip) analysis to examine the genome-wide occupancy of H3K9-me2 during all-trans-retinoic acid (ATRA)-induced differentiation of HL-60 promyelocytic leukemia cells. Using this approach, we found that KDM3B, which contains a JmjC domain, was downregulated during differentiation through the recruitment of a corepressor complex. Furthermore, KDM3B displayed histone H3K9-me1/2 demethylase activity and induced leukemogenic oncogene lmo2 expression via a synergistic interaction with CBP. Here, we found that KDM3B repressed leukemia cell differentiation and was upregulated in blood cells from acute lymphoblastic leukemia (ALL)-type leukemia patients. The combined results of this study provide evidence that the H3K9-me1/2 demethylase KDM3B might play a role in leukemogenesis via activation of lmo2 through interdependent actions with the histone acetyltransferase (HAT) complex containing CBP.

Original languageEnglish (US)
Pages (from-to)2917-2933
Number of pages17
JournalMolecular and cellular biology
Volume32
Issue number14
DOIs
StatePublished - Jul 2012

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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