Keratinocyte growth factor induces angiogenesis and protects endothelial barrier function

Paul Gillis, Ushma Savla, Olga V. Volpert, Benilde Jimenez, Christopher M. Waters, Ralph J. Panos, Noël P. Bouck*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

97 Scopus citations

Abstract

Keratinocyte growth factor (KGF), also called fibroblast growth factor-7, is widely known as a paracrine growth and differentiation factor that is produced by mesenchymal cells and has been thought to act specifically on epithelial cells. Here it is shown to affect a new cell type, the microvascular endothelial cell. At subnanomolar concentrations KGF induced in vivo neovascularization in the rat cornea. In vitro it was not effective against endothelial cells cultured from large vessels, but did act directly on those cultured from small vessels, inducing chemotaxis with an ED50 of 0.02-0.05 ng/ml, stimulating proliferation and activating mitogen activated protein kinase (MAPK). KGF also helped to maintain the barrier function of monolayers of capillary but not aortic endothelial cells, protecting against hydrogen peroxide and vascular endothelial growth factor/vascular permeability factor (VEGF/VPF) induced increases in permeability with an ED50 of 0.2-0.5 ng/ml. These newfound abilities of KGF to induce angiogenesis and to stabilize endothelial barriers suggest that it functions in microvascular tissue as it does in epithelial tissues to protect them against mild insults and to speed their repair after major damage.

Original languageEnglish (US)
Pages (from-to)2049-2057
Number of pages9
JournalJournal of cell science
Volume112
Issue number12
StatePublished - 1999

Keywords

  • Angiogenesis
  • KGF
  • Vascular permeability

ASJC Scopus subject areas

  • Cell Biology

Fingerprint Dive into the research topics of 'Keratinocyte growth factor induces angiogenesis and protects endothelial barrier function'. Together they form a unique fingerprint.

Cite this