Knockdown of hTERT and Treatment with BIBR1532 Inhibit Cell Proliferation and Invasion in Endometrial Cancer Cells

Weimin Kong, Nenan Lv, Weiya Z. Wysham, Dario R. Roque, Tongqing Zhang, Simeng Jiao, Dan Song, Jiao Chen, Victoria L. Bae-Jump, Chunxiao Zhou*

*Corresponding author for this work

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

Telomerase activity and expression of the catalytic protein hTERT are associated with cell proliferation and advanced stage in endometrial cancer. Our objective was to evaluate the effect of inhibition of hTERT by siRNA and BIBR1532 on cell growth, apoptosis and invasion in endometrial cancer cells. Knockdown of hTERT or treatment of the cells with BIBR1532 decreased telomerase activity, inhibited cell proliferation, induced apoptosis, and reduced cell invasion in Ishikawa and ECC-1 cells. Either hTERT siRNA or BIBR1532 in combination with paclitaxel promoted a synergistic inhibitory effect on cell growth through induction of Annexin V expression and a remarkable reduction in cell invasion through reduction of protein expression of MMP9, MMP2, and MMP3. Increased telomerase activity and hTERT protein expression by transfections enhanced the protein expression of MMPs and increased the cell invasion ability. BIBR1532 significantly antagonized cell invasion induced by increased hTERT expression. These findings suggest that telomerase and hTERT facilitate cell invasion via MMP family in human endometrial cancer cell.

Original languageEnglish (US)
Pages (from-to)1337-1345
Number of pages9
JournalJournal of Cancer
Volume6
Issue number12
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

Keywords

  • Apoptosis
  • Endometrial cancer
  • hTERT
  • Paclitaxel
  • Telomerase

ASJC Scopus subject areas

  • Oncology

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  • Cite this

    Kong, W., Lv, N., Wysham, W. Z., Roque, D. R., Zhang, T., Jiao, S., Song, D., Chen, J., Bae-Jump, V. L., & Zhou, C. (2015). Knockdown of hTERT and Treatment with BIBR1532 Inhibit Cell Proliferation and Invasion in Endometrial Cancer Cells. Journal of Cancer, 6(12), 1337-1345. https://doi.org/10.7150/jca.13054