Krüppel-like transcription factor 13 regulates T lymphocyte survival in vivo

Meixia Zhou, Lisa McPherson, Dongdong Feng, An Song, Chen Dong, Shu Chen Lye, Lu Zhou, Xiaoyan Shi, Yong Tae Ahn, Demin Wang, Carol Clayberger, Alan M. Krensky*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

Krüppel-like transcription factor (KLF)13, previously shown to regulate RANTES expression in vitro, is a member of the Krüppel-like family of transcription factors that controls many growth and developmental processes. To ascertain the function of KLF13 in vivo, Klf13-deficient mice were generated by gene targeting. As expected, activated T lymphocytes from Klf13 -/- mice show decreased RANTES expression. However, these mice also exhibit enlarged thymi and spleens. TUNEL, as well as spontaneous and activation-induced death assays, demonstrated that prolonged survival of Klf13-/- thymocytes was due to decreased apoptosis. Microarray analysis suggests that protection from apoptosis-inducing stimuli in Klf13 -/- thymocytes is due in part to increased expression of BCL-X L, a potent antiapoptotic factor. This finding was confirmed in splenocytes and total thymocytes by real-time quantitative PCR and Western blot as well as in CB4+CB8- single-positive thymocytes by real-time quantitative PCR. Furthermore, EMSA and luciferase reporter assays demonstrated that KLF13 binds to multiple sites within the Bcl-XL promoter and results in decreased Bcl-XL promoter activity, making KLF13 a negative regulator of BCL-XL.

Original languageEnglish (US)
Pages (from-to)5496-5504
Number of pages9
JournalJournal of Immunology
Volume178
Issue number9
DOIs
StatePublished - May 1 2007

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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