K+ waves in brain cortex visualized using a long-wavelength K+-sensing fluorescent indicator

Prashant Padmawar; Xiaoming Yao; Orin Bloch; Geoffrey T. Manley; A. S. Verkman

doi:10.1038/nmeth801

K⁺ waves in brain cortex visualized using a long-wavelength K⁺-sensing fluorescent indicator

Prashant Padmawar, Xiaoming Yao, Orin Bloch, Geoffrey T. Manley, A. S. Verkman^*

^*Corresponding author for this work

Neurological Surgery

Research output: Contribution to journal › Article › peer-review

190 Scopus citations

Abstract

We synthesized a water-soluble, long-wavelength K⁺ sensor, TAC-Red, consisting of triazacryptand coupled to 3,6-bis(dimethylamino)xanthylium, whose fluorescence increased 14-fold at 0-50 mM K⁺ with K⁺-to-Na⁺ selectivity >30. We visualized K⁺ waves in TAC-Red-stained brain cortex in mice during spreading depression, with velocity 4.4 ± 0.5 mm/min, and K⁺ release and reuptake half-times (t_1/2) of 12 ± 2 and 32 ± 4 s, respectively. Aquaporin-4 (AQP4) deletion slowed K⁺ reuptake about twofold, suggesting AQP4-dependent K⁺ uptake by astroglia.

Original language	English (US)
Pages (from-to)	825-827
Number of pages	3
Journal	Nature Methods
Volume	2
Issue number	11
DOIs	https://doi.org/10.1038/nmeth801
State	Published - Nov 2005

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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title = "K+ waves in brain cortex visualized using a long-wavelength K+-sensing fluorescent indicator",

abstract = "We synthesized a water-soluble, long-wavelength K+ sensor, TAC-Red, consisting of triazacryptand coupled to 3,6-bis(dimethylamino)xanthylium, whose fluorescence increased 14-fold at 0-50 mM K+ with K+-to-Na+ selectivity >30. We visualized K+ waves in TAC-Red-stained brain cortex in mice during spreading depression, with velocity 4.4 ± 0.5 mm/min, and K+ release and reuptake half-times (t1/2) of 12 ± 2 and 32 ± 4 s, respectively. Aquaporin-4 (AQP4) deletion slowed K+ reuptake about twofold, suggesting AQP4-dependent K+ uptake by astroglia.",

author = "Prashant Padmawar and Xiaoming Yao and Orin Bloch and Manley, {Geoffrey T.} and Verkman, {A. S.}",

note = "Funding Information: This work was supported by US National Institutes of Health grants EB00415, DK35124, EY13574, HL59198, DK72517 and HL73856, and a Research Development Program grant from the Cystic Fibrosis Foundation.",

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doi = "10.1038/nmeth801",

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journal = "Nature Methods",

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T1 - K+ waves in brain cortex visualized using a long-wavelength K+-sensing fluorescent indicator

AU - Padmawar, Prashant

AU - Yao, Xiaoming

AU - Bloch, Orin

AU - Manley, Geoffrey T.

AU - Verkman, A. S.

N1 - Funding Information: This work was supported by US National Institutes of Health grants EB00415, DK35124, EY13574, HL59198, DK72517 and HL73856, and a Research Development Program grant from the Cystic Fibrosis Foundation.

PY - 2005/11

Y1 - 2005/11

N2 - We synthesized a water-soluble, long-wavelength K+ sensor, TAC-Red, consisting of triazacryptand coupled to 3,6-bis(dimethylamino)xanthylium, whose fluorescence increased 14-fold at 0-50 mM K+ with K+-to-Na+ selectivity >30. We visualized K+ waves in TAC-Red-stained brain cortex in mice during spreading depression, with velocity 4.4 ± 0.5 mm/min, and K+ release and reuptake half-times (t1/2) of 12 ± 2 and 32 ± 4 s, respectively. Aquaporin-4 (AQP4) deletion slowed K+ reuptake about twofold, suggesting AQP4-dependent K+ uptake by astroglia.

AB - We synthesized a water-soluble, long-wavelength K+ sensor, TAC-Red, consisting of triazacryptand coupled to 3,6-bis(dimethylamino)xanthylium, whose fluorescence increased 14-fold at 0-50 mM K+ with K+-to-Na+ selectivity >30. We visualized K+ waves in TAC-Red-stained brain cortex in mice during spreading depression, with velocity 4.4 ± 0.5 mm/min, and K+ release and reuptake half-times (t1/2) of 12 ± 2 and 32 ± 4 s, respectively. Aquaporin-4 (AQP4) deletion slowed K+ reuptake about twofold, suggesting AQP4-dependent K+ uptake by astroglia.

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K⁺ waves in brain cortex visualized using a long-wavelength K⁺-sensing fluorescent indicator

Abstract

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