L-band Overhauser dynamic nuclear polarization

Sandra Garcia*, Jeffrey H. Walton, Brandon Armstrong, Songi Han, Michael J. McCarthy

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


We present the development of an Overhauser dynamic nuclear polarization (DNP) instrument at 0.04 T using 1.1 GHz (L-band) electron spin resonance frequencies (ESR) and 1.7 MHz 1H nuclear magnetic resonance frequencies. Using this home-built DNP system, the electron-nucleus coupling factor of 4-oxo-TEMPO dissolved in water was determined as 0.39 ± 0.06 at 0.04 T. The higher coupling factor obtained at this field compared to higher magnetic fields, such as 0.35 T, directly translates to higher enhancement of the NMR signal and opens up a wider time scale window for observing water dynamics interacting with macromolecular systems, including proteins, polymers or lipid vesicles. The higher enhancements obtained will facilitate the observation of water dynamics at correlation times up to 10 ns, that corresponds to more than one order of magnitude slower dynamics than accessible at 0.35 T using X-band ESR frequencies.

Original languageEnglish (US)
Pages (from-to)138-143
Number of pages6
JournalJournal of Magnetic Resonance
Issue number1
StatePublished - Mar 2010


  • Dynamic nuclear polarization
  • Electron-nucleus coupling factor
  • L-band
  • NMR

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Nuclear and High Energy Physics
  • Condensed Matter Physics


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