Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications

Emre Özkumur, James W. Needham, David A. Bergstein, Rodrigo Gonzalez, Mario Cabodi, Jonathan M. Gershoni, Bennett B. Goldberg, M. Selim Ünlü

Research output: Contribution to journalArticlepeer-review

142 Scopus citations

Abstract

Direct monitoring of primary molecular-binding interactions without the need for secondary reactants would markedly simplify and expand applications of high-throughput label-free detection methods. A simple interferometric technique is presented that monitors the optical phase difference resulting from accumulated biomolecular mass. As an example, 50 spots for each of four proteins consisting of BSA, human serum albumin, rabbit IgG, and protein G were dynamically monitored as they captured corresponding antibodies. Dynamic measurements were made at 26 pg/mm2 SD per spot and with a detectable concentration of 19 ng/ml. The presented method is particularly relevant for protein microarray analysis because it is label-free, simple, sensitive, and easily scales to high-throughput.

Original languageEnglish (US)
Pages (from-to)7988-7992
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume105
Issue number23
DOIs
StatePublished - Jun 10 2008

Keywords

  • Dynamic monitoring
  • Immunoassay
  • Interference
  • Optical biosensor
  • Protein microarray

ASJC Scopus subject areas

  • General

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