Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications

Emre Özkumur; James W. Needham; David A. Bergstein; Rodrigo Gonzalez; Mario Cabodi; Jonathan M. Gershoni; Bennett B. Goldberg; M. Selim Ünlü

doi:10.1073/pnas.0711421105

Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications

Emre Özkumur, James W. Needham, David A. Bergstein, Rodrigo Gonzalez, Mario Cabodi, Jonathan M. Gershoni, Bennett B. Goldberg, M. Selim Ünlü

Physics and Astronomy

Research output: Contribution to journal › Article › peer-review

142 Scopus citations

Abstract

Direct monitoring of primary molecular-binding interactions without the need for secondary reactants would markedly simplify and expand applications of high-throughput label-free detection methods. A simple interferometric technique is presented that monitors the optical phase difference resulting from accumulated biomolecular mass. As an example, 50 spots for each of four proteins consisting of BSA, human serum albumin, rabbit IgG, and protein G were dynamically monitored as they captured corresponding antibodies. Dynamic measurements were made at 26 pg/mm² SD per spot and with a detectable concentration of 19 ng/ml. The presented method is particularly relevant for protein microarray analysis because it is label-free, simple, sensitive, and easily scales to high-throughput.

Original language	English (US)
Pages (from-to)	7988-7992
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	105
Issue number	23
DOIs	https://doi.org/10.1073/pnas.0711421105
State	Published - Jun 10 2008

Keywords

Dynamic monitoring
Immunoassay
Interference
Optical biosensor
Protein microarray

ASJC Scopus subject areas

General

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10.1073/pnas.0711421105

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Özkumur, E., Needham, J. W., Bergstein, D. A., Gonzalez, R., Cabodi, M., Gershoni, J. M., Goldberg, B. B., & Ünlü, M. S. (2008). Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications. Proceedings of the National Academy of Sciences of the United States of America, 105(23), 7988-7992. https://doi.org/10.1073/pnas.0711421105

Özkumur, Emre ; Needham, James W. ; Bergstein, David A. ; Gonzalez, Rodrigo ; Cabodi, Mario ; Gershoni, Jonathan M. ; Goldberg, Bennett B. ; Ünlü, M. Selim. / Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications. In: Proceedings of the National Academy of Sciences of the United States of America. 2008 ; Vol. 105, No. 23. pp. 7988-7992.

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Özkumur, E, Needham, JW, Bergstein, DA, Gonzalez, R, Cabodi, M, Gershoni, JM, Goldberg, BB & Ünlü, MS 2008, 'Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications', Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 23, pp. 7988-7992. https://doi.org/10.1073/pnas.0711421105

Label-free and dynamic detection of biomolecular interactions for high-throughput microarray applications. / Özkumur, Emre; Needham, James W.; Bergstein, David A.; Gonzalez, Rodrigo; Cabodi, Mario; Gershoni, Jonathan M.; Goldberg, Bennett B.; Ünlü, M. Selim.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, No. 23, 10.06.2008, p. 7988-7992.

Research output: Contribution to journal › Article › peer-review

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