Label-free detection of protein-protein interactions on biochips

Woon Seok Yeo; Dal Hee Min; Robert W. Hsieh; Geoffrey L. Greene; Milan Mrksich

doi:10.1002/anie.200501363

Label-free detection of protein-protein interactions on biochips

Woon Seok Yeo^*, Dal Hee Min, Robert W. Hsieh, Geoffrey L. Greene, Milan Mrksich

^*Corresponding author for this work

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

(Figure Presented) Eliminating the middle-man: The combination of MALDI-TOF mass spectrometry and self-assembled monolayers on gold (SAMDI) offers a new detection method for protein-ligand (shown) and protein-protein interactions. Proteins and protein complexes are selectively bound to biochips and then directly analyzed by MALDI-TOF MS without the need for labels.

Original language	English (US)
Pages (from-to)	5480-5483
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	44
Issue number	34
DOIs	https://doi.org/10.1002/anie.200501363
State	Published - Sep 2 2005

Keywords

Bioinformatics
Immobilization
Mass spectrometry
Monolayers
Proteins

ASJC Scopus subject areas

General Chemistry
Catalysis

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10.1002/anie.200501363

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title = "Label-free detection of protein-protein interactions on biochips",

abstract = "(Figure Presented) Eliminating the middle-man: The combination of MALDI-TOF mass spectrometry and self-assembled monolayers on gold (SAMDI) offers a new detection method for protein-ligand (shown) and protein-protein interactions. Proteins and protein complexes are selectively bound to biochips and then directly analyzed by MALDI-TOF MS without the need for labels.",

keywords = "Bioinformatics, Immobilization, Mass spectrometry, Monolayers, Proteins",

author = "Yeo, {Woon Seok} and Min, {Dal Hee} and Hsieh, {Robert W.} and Greene, {Geoffrey L.} and Milan Mrksich",

year = "2005",

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doi = "10.1002/anie.200501363",

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T1 - Label-free detection of protein-protein interactions on biochips

AU - Yeo, Woon Seok

AU - Min, Dal Hee

AU - Hsieh, Robert W.

AU - Greene, Geoffrey L.

AU - Mrksich, Milan

PY - 2005/9/2

Y1 - 2005/9/2

N2 - (Figure Presented) Eliminating the middle-man: The combination of MALDI-TOF mass spectrometry and self-assembled monolayers on gold (SAMDI) offers a new detection method for protein-ligand (shown) and protein-protein interactions. Proteins and protein complexes are selectively bound to biochips and then directly analyzed by MALDI-TOF MS without the need for labels.

AB - (Figure Presented) Eliminating the middle-man: The combination of MALDI-TOF mass spectrometry and self-assembled monolayers on gold (SAMDI) offers a new detection method for protein-ligand (shown) and protein-protein interactions. Proteins and protein complexes are selectively bound to biochips and then directly analyzed by MALDI-TOF MS without the need for labels.

KW - Bioinformatics

KW - Immobilization

KW - Mass spectrometry

KW - Monolayers

KW - Proteins

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DO - 10.1002/anie.200501363

M3 - Article

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JO - Angewandte Chemie - International Edition

JF - Angewandte Chemie - International Edition

IS - 34

ER -

Label-free detection of protein-protein interactions on biochips

Abstract

Keywords

ASJC Scopus subject areas

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