TY - JOUR
T1 - Label free localization of nanoparticles in live cancer cells using spectroscopic microscopy
AU - Spicer, Graham L.C.
AU - Almassalha, Luay
AU - Martinez, Ignacio A.
AU - Ellis, Ronald
AU - Chandler, John E.
AU - Gladstein, Scott
AU - Zhang, Di
AU - Nguyen, The Quyen
AU - Feder, Seth
AU - Subramanian, Hariharan
AU - De La Rica, Roberto
AU - Thompson, Sebastian A.
AU - Backman, Vadim
PY - 2018/10/28
Y1 - 2018/10/28
N2 - Gold nanoparticles (GNPs) have become essential tools used in nanobiotechnology due to their tunable plasmonic properties and low toxicity in biological samples. Among the available approaches for imaging GNPs internalized by cells, hyperspectral techniques stand out due to their ability to simultaneously image and perform spectral analysis of GNPs. Here, we present a study utilizing a recently introduced hyperspectral imaging technique, live-cell PWS, for the imaging, tracking, and spectral analysis of GNPs in live cancer cells. Using principal components analysis, the extracellular or intracellular localization of the GNPs can be determined without the use of exogenous labels. This technique uses wide-field white light, assuring minimal toxicity and suitable signal-to-noise ratio for spectral and temporal resolution of backscattered signal from GNPs and local cellular structures. The application of live-cell PWS introduced here could make a great impact in nanomedicine and nanotechnology by giving new insights into GNP internalization and intracellular trafficking.
AB - Gold nanoparticles (GNPs) have become essential tools used in nanobiotechnology due to their tunable plasmonic properties and low toxicity in biological samples. Among the available approaches for imaging GNPs internalized by cells, hyperspectral techniques stand out due to their ability to simultaneously image and perform spectral analysis of GNPs. Here, we present a study utilizing a recently introduced hyperspectral imaging technique, live-cell PWS, for the imaging, tracking, and spectral analysis of GNPs in live cancer cells. Using principal components analysis, the extracellular or intracellular localization of the GNPs can be determined without the use of exogenous labels. This technique uses wide-field white light, assuring minimal toxicity and suitable signal-to-noise ratio for spectral and temporal resolution of backscattered signal from GNPs and local cellular structures. The application of live-cell PWS introduced here could make a great impact in nanomedicine and nanotechnology by giving new insights into GNP internalization and intracellular trafficking.
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U2 - 10.1039/c8nr07481j
DO - 10.1039/c8nr07481j
M3 - Article
C2 - 30298892
AN - SCOPUS:85055070927
VL - 10
SP - 19125
EP - 19130
JO - Nanoscale
JF - Nanoscale
SN - 2040-3364
IS - 40
ER -