Label-free quantitation for clinical proteomics

Robert Moulder, Young Ah Goo, David R. Goodlett

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

Label-free quantification (LFQ) has emerged as a viable option for quantitative LC-MS/MS-based proteomic analyses for use on the scale of hundreds of samples such as are encountered in clinical analysis. Notably, sample preparation, sample loading, HPLC separations, and mass spectrometric performance must be highly reproducible for this approach to be effective. The following protocols describe the key steps in the methods related to sample preparation and analysis for LC-MS/MS-based label-free quantitation using standard data-dependent acquisition.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc
Pages65-76
Number of pages12
DOIs
StatePublished - Feb 1 2016

Publication series

NameMethods in Molecular Biology
Volume1410
ISSN (Print)1064-3745

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Keywords

  • Area under the curve (AUC)
  • Label-free quantification
  • Mass spectrometry
  • Proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Moulder, R., Goo, Y. A., & Goodlett, D. R. (2016). Label-free quantitation for clinical proteomics. In Methods in Molecular Biology (pp. 65-76). (Methods in Molecular Biology; Vol. 1410). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3524-6_4