Labeling of creatine phosphokinase without loss of enzyme activity

Victor S. Fang*, H. W. Cho, Herbert Y. Meltzer

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Creatine phosphokinase (CPK) loses enzyme activity after direct iodination by the chloramine-T, thallic trichloride and lactoperoxidase methods. Radioiodination of CPK without loss of enzyme activity can be achieved by Bolton-Hunter reagent which conjugates at the N-terminal or e{open}-lysyl NH2 group of the protein.

Original languageEnglish (US)
Pages (from-to)413-419
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume65
Issue number1
DOIs
StatePublished - Jul 8 1975

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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