TY - JOUR
T1 - Laboratory and clinical features of abnormal macroenzymes found in human sera
AU - Moriyama, Takanori
AU - Tamura, Shogo
AU - Nakano, Keiichi
AU - Otsuka, Kohei
AU - Shigemura, Masahiko
AU - Honma, Naoyuki
N1 - Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - We report the analysis of unusual macroenzymes, performed in our laboratory, and review the relevant literature. In particular, we focused on macro AST, macroamylase, macro LD and macro CK. Macroenzymes are seen in healthy subjects, but can also be related to disease; thus, accurate detection is useful in day-to-day clinical practice. The macroenzyme is thought to be a specific antigen-antibody complex from the following findings: (1) the complex could be dissociated under acidic pH levels; (2) binding specificity of immunoglobulin in the complex was observed; (3) the binding site of immunoglobulin in the complex was Fab portion; and (4) the maternal IgG involved with macroenzyme was transferred to her children. This article is part of a Special Issue entitled: Medical Proteomics.
AB - We report the analysis of unusual macroenzymes, performed in our laboratory, and review the relevant literature. In particular, we focused on macro AST, macroamylase, macro LD and macro CK. Macroenzymes are seen in healthy subjects, but can also be related to disease; thus, accurate detection is useful in day-to-day clinical practice. The macroenzyme is thought to be a specific antigen-antibody complex from the following findings: (1) the complex could be dissociated under acidic pH levels; (2) binding specificity of immunoglobulin in the complex was observed; (3) the binding site of immunoglobulin in the complex was Fab portion; and (4) the maternal IgG involved with macroenzyme was transferred to her children. This article is part of a Special Issue entitled: Medical Proteomics.
KW - Macro aspartate aminotransferase (AST)
KW - Macro creatine kinase (CK)
KW - Macro lactate dehydrogenase (LD)
KW - Macroamylase
KW - Macroenzyme
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U2 - 10.1016/j.bbapap.2014.10.015
DO - 10.1016/j.bbapap.2014.10.015
M3 - Review article
C2 - 25545221
AN - SCOPUS:84939974290
SN - 1570-9639
VL - 1854
SP - 658
EP - 667
JO - Biochimica et Biophysica Acta - Proteins and Proteomics
JF - Biochimica et Biophysica Acta - Proteins and Proteomics
IS - 6
ER -