TY - JOUR
T1 - Lack of Association of C677T Methylenetetrahydrofolate Reductase Polymorphism with Breast Cancer Risk in Mali
AU - Diakite, Brehima
AU - Kassogue, Yaya
AU - Maiga, Mamoudou
AU - Dolo, Guimogo
AU - Kassogue, Oumar
AU - Holl, Jane L.
AU - Joyce, Brian
AU - Wang, Jun
AU - Cisse, Kadidiatou
AU - Diarra, Fousseyni
AU - Keita, Mamadou L.
AU - Traore, Cheick B.
AU - Kamate, Bakarou
AU - Sissoko, Sidi B.
AU - Coulibaly, Bourama
AU - Sissoko, Adama S.
AU - Traore, Drissa
AU - Sidibe, Fatoumata M.
AU - Bah, Sekou
AU - Teguete, Ibrahim
AU - Ly, Madani
AU - Nadifi, Sellama
AU - Dehbi, Hind
AU - Kim, Kyeezu
AU - Murphy, Robert
AU - Hou, Lifang
N1 - Publisher Copyright:
© 2023 Brehima Diakite et al.
PY - 2023
Y1 - 2023
N2 - Methylenetetrahydrofolate reductase (MTHFR) plays a major role in the metabolism of folates and homocysteine, which in turn can affect gene expression and ultimately promote the development of breast cancer. Thus, mutations in the MTHFR gene could influence homocysteine, methionine, and S-adenosylmethionine levels and, indirectly, nucleotide levels. Imbalance in methionine and S-adenosylmethionine synthesis affects protein synthesis and methylation. These changes, which affect gene expression, may ultimately promote the development of breast cancer. We therefore hypothesized that such mutations could also play an important role in the occurrence and pathogenesis of breast cancer in a Malian population. In this study, we used the PCR-RFLP technique to identify the different genotypic profiles of the C677T MTHFR polymorphism in 127 breast cancer women and 160 healthy controls. The genotypic distribution of the C677T polymorphism in breast cancer cases was 88.2% for CC, 11.0% for CT, and 0.8% for TT. Healthy controls showed a similar distribution with 90.6% for CC, 8.8% for CT, and 0.6% for TT. We found no statistical association between the C677T polymorphism and breast cancer risk for the codominant models CT and TT p>0.05. The same trend was observed when the analysis was extended to other genetic models, including dominant (p = 0.50), recessive (p = 0.87), and additive (p = 0.50) models. The C677T polymorphism of MTHFR gene did not influence the risk of breast cancer in the Malian samples.
AB - Methylenetetrahydrofolate reductase (MTHFR) plays a major role in the metabolism of folates and homocysteine, which in turn can affect gene expression and ultimately promote the development of breast cancer. Thus, mutations in the MTHFR gene could influence homocysteine, methionine, and S-adenosylmethionine levels and, indirectly, nucleotide levels. Imbalance in methionine and S-adenosylmethionine synthesis affects protein synthesis and methylation. These changes, which affect gene expression, may ultimately promote the development of breast cancer. We therefore hypothesized that such mutations could also play an important role in the occurrence and pathogenesis of breast cancer in a Malian population. In this study, we used the PCR-RFLP technique to identify the different genotypic profiles of the C677T MTHFR polymorphism in 127 breast cancer women and 160 healthy controls. The genotypic distribution of the C677T polymorphism in breast cancer cases was 88.2% for CC, 11.0% for CT, and 0.8% for TT. Healthy controls showed a similar distribution with 90.6% for CC, 8.8% for CT, and 0.6% for TT. We found no statistical association between the C677T polymorphism and breast cancer risk for the codominant models CT and TT p>0.05. The same trend was observed when the analysis was extended to other genetic models, including dominant (p = 0.50), recessive (p = 0.87), and additive (p = 0.50) models. The C677T polymorphism of MTHFR gene did not influence the risk of breast cancer in the Malian samples.
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U2 - 10.1155/2023/4683831
DO - 10.1155/2023/4683831
M3 - Article
C2 - 36721432
AN - SCOPUS:85147186845
SN - 0016-6723
VL - 2023
JO - Genetics Research
JF - Genetics Research
M1 - 4683831
ER -