Abstract
On account of its excellent resolution and high throughput, cryoSEM imaging has recently seen resurgence. In this work, we report on the development of cryogenic triple ion gun milling (CryoTIGM™), a broad ion beam milling technique for cryo-planing of vitrified, frozen-hydrated specimens. We find that sections prepared with CryoTIGM™ are smooth over exceptionally large areas (∼700,000 μm2), and reveal ultrastructural details in similar or better quality than freeze-fractured samples.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1616-1621 |
| Number of pages | 6 |
| Journal | Microscopy and Microanalysis |
| Volume | 21 |
| Issue number | 6 |
| DOIs | |
| State | Published - Oct 12 2015 |
Keywords
- cryo-planning
- cryoSEM
- freeze fracture
- high pressure freezing
- ultrastructure
ASJC Scopus subject areas
- Instrumentation
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Chang, I. Y. T., & Joester, D. (2015). Large Area Cryo-Planing of Vitrified Samples Using Broad-Beam Ion Milling. Microscopy and Microanalysis, 21(6), 1616-1621. https://doi.org/10.1017/S143192761501510X