Large Area Cryo-Planing of Vitrified Samples Using Broad-Beam Ion Milling

Irene Y.T. Chang; Derk Joester

doi:10.1017/S143192761501510X

Large Area Cryo-Planing of Vitrified Samples Using Broad-Beam Ion Milling

Irene Y.T. Chang, Derk Joester^*

^*Corresponding author for this work

Materials Science and Engineering

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

On account of its excellent resolution and high throughput, cryoSEM imaging has recently seen resurgence. In this work, we report on the development of cryogenic triple ion gun milling (CryoTIGM^™), a broad ion beam milling technique for cryo-planing of vitrified, frozen-hydrated specimens. We find that sections prepared with CryoTIGM^™ are smooth over exceptionally large areas (∼700,000 μm²), and reveal ultrastructural details in similar or better quality than freeze-fractured samples.

Original language	English (US)
Pages (from-to)	1616-1621
Number of pages	6
Journal	Microscopy and Microanalysis
Volume	21
Issue number	6
DOIs	https://doi.org/10.1017/S143192761501510X
State	Published - Oct 12 2015

Keywords

cryo-planning
cryoSEM
freeze fracture
high pressure freezing
ultrastructure

ASJC Scopus subject areas

Instrumentation

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title = "Large Area Cryo-Planing of Vitrified Samples Using Broad-Beam Ion Milling",

abstract = "On account of its excellent resolution and high throughput, cryoSEM imaging has recently seen resurgence. In this work, we report on the development of cryogenic triple ion gun milling (CryoTIGM{\texttrademark}), a broad ion beam milling technique for cryo-planing of vitrified, frozen-hydrated specimens. We find that sections prepared with CryoTIGM{\texttrademark} are smooth over exceptionally large areas (∼700,000 μm2), and reveal ultrastructural details in similar or better quality than freeze-fractured samples.",

keywords = "cryo-planning, cryoSEM, freeze fracture, high pressure freezing, ultrastructure",

author = "Chang, {Irene Y.T.} and Derk Joester",

note = "Funding Information: The NSF (MRI-1229693, DMR-1106208), the Northwestern University Materials Research Center (DMR-1121262), and the International Institute for Nanotechnology (IIN) in part supported this work. Experiments were performed at NUANCE-EPIC and OMM, Northwestern University core facilities that are supported by the MRSEC program (NSF DMR-1121262), the IIN; and the State of Illinois. The authors thank Dr. Reiner Bleher for discussion.",

year = "2015",

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doi = "10.1017/S143192761501510X",

language = "English (US)",

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AU - Joester, Derk

N1 - Funding Information: The NSF (MRI-1229693, DMR-1106208), the Northwestern University Materials Research Center (DMR-1121262), and the International Institute for Nanotechnology (IIN) in part supported this work. Experiments were performed at NUANCE-EPIC and OMM, Northwestern University core facilities that are supported by the MRSEC program (NSF DMR-1121262), the IIN; and the State of Illinois. The authors thank Dr. Reiner Bleher for discussion.

PY - 2015/10/12

Y1 - 2015/10/12

N2 - On account of its excellent resolution and high throughput, cryoSEM imaging has recently seen resurgence. In this work, we report on the development of cryogenic triple ion gun milling (CryoTIGM™), a broad ion beam milling technique for cryo-planing of vitrified, frozen-hydrated specimens. We find that sections prepared with CryoTIGM™ are smooth over exceptionally large areas (∼700,000 μm2), and reveal ultrastructural details in similar or better quality than freeze-fractured samples.

AB - On account of its excellent resolution and high throughput, cryoSEM imaging has recently seen resurgence. In this work, we report on the development of cryogenic triple ion gun milling (CryoTIGM™), a broad ion beam milling technique for cryo-planing of vitrified, frozen-hydrated specimens. We find that sections prepared with CryoTIGM™ are smooth over exceptionally large areas (∼700,000 μm2), and reveal ultrastructural details in similar or better quality than freeze-fractured samples.

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KW - freeze fracture

KW - high pressure freezing

KW - ultrastructure

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Large Area Cryo-Planing of Vitrified Samples Using Broad-Beam Ion Milling

Abstract

Keywords

ASJC Scopus subject areas

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