Abstract
On account of its excellent resolution and high throughput, cryoSEM imaging has recently seen resurgence. In this work, we report on the development of cryogenic triple ion gun milling (CryoTIGM™), a broad ion beam milling technique for cryo-planing of vitrified, frozen-hydrated specimens. We find that sections prepared with CryoTIGM™ are smooth over exceptionally large areas (∼700,000 μm2), and reveal ultrastructural details in similar or better quality than freeze-fractured samples.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1616-1621 |
| Number of pages | 6 |
| Journal | Microscopy and Microanalysis |
| Volume | 21 |
| Issue number | 6 |
| DOIs | |
| State | Published - Oct 12 2015 |
Funding
The NSF (MRI-1229693, DMR-1106208), the Northwestern University Materials Research Center (DMR-1121262), and the International Institute for Nanotechnology (IIN) in part supported this work. Experiments were performed at NUANCE-EPIC and OMM, Northwestern University core facilities that are supported by the MRSEC program (NSF DMR-1121262), the IIN; and the State of Illinois. The authors thank Dr. Reiner Bleher for discussion.
Keywords
- cryo-planning
- cryoSEM
- freeze fracture
- high pressure freezing
- ultrastructure
ASJC Scopus subject areas
- Instrumentation